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iPSC Culture Protocol Videos
Watch our iPSC culture protocol videos to learn how to culture iPSCs and iPSC-derived neural cells.
English, French, Portuguese, and Spanish videos are available now, with more languages coming soon!
At the EDDU, we developed a method with microfabricated disk (EB disk-948 from eNUVIO) to scale up the generation of hMOs (human midbrain organoids). This opens up the possibility to generate larger numbers of hMOs, in a manner that minimizes the amount of labor required, while decreasing variability and maintaining the viability of these hMOs over time.
This video will describe the methodology to generate hMOs from a single EB-Disk containing 948 microwells.
The Neuro’s EDDU is also pleased to share our updated protocol for generating dopaminergic neurons from iPSCs through the use of a Microfabricated EB Disk. You can access the protocol here: https://zenodo.org/record/8256637
Latest Publications
To access all of the EDDU's publications, please visit our Community and Resource Hub.
Single Cell RNAseq to identify subpopulations of glial progenitors in iPSC-derived oligodendroglial lineage cultures
April 2025
Gabriela J Blaszczyk et al.
An Inducible Luminescent System to Explore Parkinson’s Disease-Associated Genes
August 2024
Anelya Gandy et al.
Preprints
Mutations in SOD1 induce ALS-related phenotypes in 3D iPSC-derived motor neuron (MN) spheroids
January 2025
María José Castellanos-Montiel et al.
Pro-Inflammatory Molecules Implicated in Multiple Sclerosis Divert the Development of Human Oligodendrocyte Lineage Cells
December 2024
Gabriela J Blaszczyk et al.
How to Access iPSCs
At the EDDU we have a catalogue of iPSC lines from healthy individuals and from patients with neurological disorders that can be freely accessed for a small fee. Our cells are for experimental use only.
How to access the iPSC catalogue:
- Click “Open data access” as login
- Select at the top-left “Open data” tab
- Click “iPSC catalogue”
To access the cells, please cbig.mni [at] mcgill.ca (C-BIG). Click here for further instructions on accessing cells.
To learn more about how the cells are made or for general inquiries, please email xiuqing.chen [at] mcgill.ca (Carol Chen) and taylor.goldsmith [at] mcgill.ca ( Taylor Goldsmith).
Visit our Community and Resource Hub
To access the EDDU’s Open Science resources including our publications, presentations, protocols, and software please visit our Community and Resource Hub.
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