Watch our iPSC culture protocol videos to learn how to culture iPSCs and iPSC-derived neural cells.
English, French, Portuguese, and Spanish videos are available now, with more languages coming soon!
Assessing induced-pluripotent cells (iPSC) morphology is important to guarantee that the quality of iPSC cultures is high and to ensure that these cells keep their pluripotent state. It is important to do this step every time before manipulating the cells. In this video, we will discuss the morphological characteristics of healthy-looking iPSC colonies and how to spot areas of spontaneous differentiation.
Read our publication about iPSC quality control here.
Funding from Tanenbaum Open Science Institute (TOSI) at The Neuro helped make this video possible.
To access all of the EDDU's publications, please visit our Data Portal.
Generation of homozygous PRKN, PINK1, and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing
Carol X.-Q. Chen et al.
A light-inducible protein clustering system for in-vivo analysis of α-synuclein aggregation in Parkinson disease
Morgan Bérard et al.
The identification of potent and selective antibodies of the Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation.
Walaa Alshafie et al.
Rapid micropinocytic transfer of α-synuclein to lysosomes
Armin Bayati et al.
At the EDDU we have a catalogue of iPSC lines from healthy individuals and from patients with neurological disorders that can be freely accessed for a small fee. Our cells are for experimental use only.
To access the cells, please contact cbig.mni [at] mcgill.ca (C-BIG). Click here for further instructions on accessing cells.
To learn more about how the cells are made or for general inquiries, contact us at neuroeddu.mni [at] mcgill.ca.
To access the EDDU’s Open Science resources including our publications, presentations, protocols, and software please visit our Data Portal.