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iPSC Culture Protocol Videos

Watch our iPSC culture protocol videos to learn how to culture iPSCs and iPSC-derived neural cells.

English, French, Portuguese, and Spanish videos are available now, with more languages coming soon!

 

Assessing induced-pluripotent cells (iPSC) morphology is important to guarantee that the quality of iPSC cultures is high and to ensure that these cells keep their pluripotent state. It is important to do this step every time before manipulating the cells. In this video, we will discuss the morphological characteristics of healthy-looking iPSC colonies and how to spot areas of spontaneous differentiation. 

Read our publication about iPSC quality control here.

Funding from Tanenbaum Open Science Institute (TOSI) at The Neuro helped make this video possible.

 


Latest Publications

To access all of the EDDU's publications, please visit our Data Portal

 

Rapid macropinocytic transfer of α-synuclein to lysosomes

July 2022

Armin Bayati et al. 

Graphical abstract

Read More

Generation of homozygous PRKN, PINK1, and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing

July 2022

Carol X.-Q. Chen et al. 

Fig. 1. Characterization of isogenic PRKN-KO, PINK1-KO, and PINK1-KO/PRKN-KO iPSC lines.

Read More

 

 


Preprints

 

The identification of potent and selective antibodies of the Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation.

June 2022

Walaa Alshafie et al. 

Serine/threonine-protein kinase TBK1 antibody screening by immunoblot

Read More

Rapid micropinocytic transfer of α-synuclein to lysosomes

May 2022

Armin Bayati et al.

PFF remain in lysosomes for at least 7 days in dopaminergic neurons and astrocytes.

Read More

 

 


How to Access iPSCs

At the EDDU we have a catalogue of iPSC lines from healthy individuals and from patients with neurological disorders that can be freely accessed for a small fee. Our cells are for experimental use only.

To access the cells, please contact cbig.mni [at] mcgill.ca (C-BIG). Click here for further instructions on accessing cells.

To learn more about how the cells are made or for general inquiries, contact us at neuroeddu.mni [at] mcgill.ca.

View the iPSC Catalogue

 


Visit our Data Portal

To access the EDDU’s Open Science resources including our publications, presentations, protocols, and software please visit our Data Portal.

Access The Neuro EDDU Data Portal

 

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We are part of Open Science at The Neuro

We believe in freely sharing data and materials to speed up drug discovery and engage in the following Open Science activities:

  1. Open data - Publishing in open-access journals
  2. Open access - Sharing protocols and providing training
  3. Open intellectual property - Promoting open partnerships with industry
  4. Open sharing - Making our reagents available

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The Neuro (Montreal Neurological Institute-Hospital) is a bilingual academic healthcare institution. We are a McGill research and teaching institute; delivering high-quality patient care, as part of the Neuroscience Mission of the McGill University Health Centre. We are proud to be a Killam Institution, supported by the Killam Trusts.

 

 

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