Mapping the tadpole brain in the land of the homunculus

New study expands on Dr. Wilder Penfield’s groundbreaking discovery of the human brain's sensory maps

Just under a century ago, the great neurosurgeon Dr. Wilder Penfield, founder of The Neuro (Montreal Neurological Institute-Hospital), pioneered the treatment of epilepsy by surgical excision of the seizure focus from the cerebral cortex of patients.

While carrying out these surgeries, which came to be known as the “Montreal procedure”, Penfield and his fellow neurosurgeons attempted to minimize damage by systematically stimulating the cortex to map out sites surrounding the presumptive lesion. By this process of systematic functional mapping of the cortex, Penfield discovered the orderly representations of sensory inputs and motor outputs that are mapped across the cortical surface. This discovery gave birth to the so-called sensory and motor homunculi, distorted “little men” representing the relative cortical territory allotted to each body part, firmly establishing the existence of functional sensory maps in the brain.

A homunculus

Around the same time, future Nobel laureate Roger Sperry, still a young postdoctoral researcher, had become fascinated by the formation of sensory maps in the visual system. Sperry used a number of different species, including the African claw-toed frog Xenopus laevis, to examine how regenerating axonal projections from the retina to the primary visual centre in the amphibian brain, called the optic tectum, achieved their precise organization in the target. Neurons from across the retina extend long axonal projections through the optic nerve to the brain where they remarkably reorganize themselves back into the same relative layout they had within the eye, creating a “retinotopic” map that effectively recreates within the brain the visual scene viewed by the eye. In a series of famous experiments, Sperry and co-workers lesioned parts of the eye, even going so far as to rotate eyes by 180 degrees, to demonstrate that retinal axons find their destinations using prespecified molecular address codes that he referred to as “chemoaffinity” cues.

A legacy furthered

A recent study by PhD candidate Vanessa Li and colleagues at The Neuro has revisited the early formation of visual system maps in the Xenopus optic tectum, using optogenetic technologies to follow the emergence of retinotopic maps at subcellular resolution in the brains of living, developing Xenopus tadpoles.

The researchers grew genetically modified Xenopus tadpoles whose neurons were made to express the fluorescent protein GCaMP6s, which increases its brightness when neurons are activated. They then imaged their brains non-invasively using a high-speed two-photon microscope, capable of creating three-dimensional reconstructions of the entire brain, akin to the brain sectioning performed in an MRI scan, but at much finer resolution. The researchers presented visual stimuli to the tadpoles and asked what cells in their brains had been activated by stimulation in different parts of the visual field. Using this data, they generated cellular-resolution retinotopic maps in the developing brain starting as early as just one day after the projections from the eye first arrived in the brain and following them in the same animals as they developed over subsequent days.

Tadpole in green staining

Their paper, published in the February 14 issue of Proceedings of the National Academy of Sciences, confirms researchers’ long-held notion that maps in the brain become increasingly precise over time as the brain grows, but also reveals some unexpected twists. One surprising finding was how well-organized certain aspects of the visual maps in the brain are already at their initial emergence.

Another impressive finding was the observation that the retinotopic maps physically rotate within the developing brain over several days. These results highlight just how plastic and malleable the young brain can be, even despite the presence of the chemoaffinity cues that specify the initial connectivity.

Click here to read the full paper.

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The Neuro (Montreal Neurological Institute-Hospital) is a bilingual academic healthcare institution. We are a McGill research and teaching institute; delivering high-quality patient care, as part of the Neuroscience Mission of the McGill University Health Centre. We are proud to be a Killam Institution, supported by the Killam Trusts.

 

 

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