Standard Operating Procedures (SOPs)

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Standard Operating Procedures (SOPs) provide a detailed description of commonly used procedures.

Note that the use of the words ‘should’ and ‘must’ in these SOPs is consistent with their defined use in Canadian Council on Animal Care (CCAC) guidelines. The term 'should' indicates an obligation, for which any exceptions must be justified to, and approved by, an Animal Care Committee. The term 'must' indicates a mandatory requirement.

Any deviation from the approved procedures must be clearly described and justified in the Animal Use Protocol application in AMS. Approval of the protocol indicates approval of the deviation from the SOP for that project only.

The following Standard Operating Procedures (SOPs) were created and/or revised by the Veterinary Committee and approved by the Animal Policy and Welfare Oversight Committee (APWOC).

If you have questions or have a recommendation to make, please send an animalcare [at] mcgill.ca (email) or talk to the Veterinarian in your area.

SOPs

Controlled Substances

SOP 401 on Cannabis and Controlled Substances.

SOP 101-Rodent Analgesia
SOP 103-NHP Analgesia
SOP 106-Swine Analgesia
SOP 107-Bird Analgesia
SOP 108-Fish Aquatic Amphibian Reptile Analgesia

SOP 110 - Mouse Anesthesia	SOP 119 - Swine Anesthesia
SOP 111 - Rat Anesthesia	SOP 120 - Rodent Anesthesia Protocol Lung Physiology Measurements
SOP 115 - NHP Anesthesia	SOP 121 - Bird Anesthesia
SOP 118 - Fish and Aquatic Amphibian Anesthesia

SOP 201 - Rodent Surgery	SOP 205 - Rodent Tumor Resection
SOP 202 - Rodent Stereotaxic Surgery	SOP 206 - Rodent Ovariectomy
SOP 203 - Large Animal Surgery	SOP 207 - Rodent Castration
SOP 204 - Xenopus Egg and Oocyte Collection	SOP 208 - Rodent Mammary Fat Pad Injection

SOP 301 - Rodent Euthanasia	SOP 304 - Avian Euthanasia
SOP 302 - Large Animal Euthanasia	SOP 305 - Transcardiac Perfusion
SOP 303 - Fish and Aquatic Amphibian Euthanasia

McGill's EHS SOP 401 - Controlled Substances	SOP 410 - Humane Intervention Points
SOP 402 - Tissue Collection for Genotyping - Mice	SOP 412 - Humane Intervention Points for Aging Rodents
SOP 403 - Guidelines Blood Collection Volumes and Frequency	SOP 413 - Tissue Collection for Genotyping - Rats
SOP 404 - Substance Administration	SOP 414 - Rodent Algesiometric Testing
SOP 405 - Monoclonal Antibody Production - Mice	SOP 415 - Humane Intervention Points for Rodent Cancer Models
SOP 406 - Polyclonal Antibody Production - Rabbits	SOP 417 - Food and Water Restriction in Rodents
SOP 407 - Polyclonal Antibody Production - Rats	SOP 418 - Humane Intervention Points for Animals Used in Training
SOP 409 - Maintaining Clinical Records	SOP 419 - Humane Intervention Points for Rodents Models Involving Paralysis
	SOP 420 - Estrous Cycle Assessment in Rodents
This Monitoring Log is to be prepared by the Facility Animal Care Committee as per the approved Animal Use Protocol:  Monitoring log TEMPLATE
CMARC NHP Activity Tracking and Procedures Requiring Anesthesia Log

SOP 501- Animal Transport and Use Outside of Animal Facilities	SOP 522 - Environmental Enrichment Program for Fish
SOP 502 - Environmental Enrichment Program for Rodents	SOP 523 - Environmental Enrichment Program for Amphibians
SOP 504 - Environmental Enrichment Program for Rabbits	SOP 525 - Cat Husbandry
SOP 505 - Environmental Enrichment Program for Hamsters	SOP 526 - Environmental Enrichment Program for Cats
SOP 506 - Environmental Enrichment Program for Guinea Pigs	SOP 527 - Finch Husbandry
SOP 507 - Environmental Enrichment Program for NHPs	SOP 528 - Environmental Enrichment Program for Birds
SOP 508 - Rodent Husbandry	SOP 531 - Animal Acquisition Receiving and Acclimation
SOP 509 - Rodent Handling and Restraint	SOP 532 - Vermin Control Program
SOP 513 - Marmoset Husbandry	SOP 533 - Microbiological Monitoring Program
SOP 515 - Marmoset Feeding	SOP 536 - Procedure Room Maintenance
SOP 516 - Marmoset Cage Changing	SOP 537 - Working in Laminar Flow Hood or Animal Transfer Station or Biological Safety Cabinet
SOP 517 - Marmoset Room Sanitation	SOP 540 - Operating Room Maintenance
SOP 518 - Transportation of NHPs Between Facilities	SOP 541 - Vendor Approval for Animal Feed and Bedding Suppliers
SOP 519 - Aquatic Animal Husbandry
SOP 521 - Macaque Husbandry

SOP 601 - Veterinary Care Program	SOP 614 - Guidelines on Wire-Bottom Caging for Rodents
SOP 602 - Rodent Health Monitoring Program	SOP 615 - Rodent Irradiation
SOP 603 - Rodent Quarantine Program	SOP 616 - Rodent Identification
SOP 604 - Bioexclusion Levels for Rodents	SOP 619 - Common Rodent Treatment
SOP 605 - Rodent Import	SOP 622 - Marmoset Health Monitoring Program
SOP 606 - Reporting Concerns Regarding Animal Care and Use	SOP 623 - Marmoset Breeding Colony Management
SOP 607 - Long-term Housing	SOP 626 - Macaque Health Monitoring Program
SOP 608 - Mouse Breeding Colony Management	SOP 631 - Macaque Herpes B Testing
SOP 609 - Rat Breeding Colony Management	SOP 632 - Animal Welfare Assessment
SOP 610 - Testing of Biological Material	SOP 636 - Storage and Transport of Primate Tissues
SOP 611 - Excluded Pathogens - Rodent Facilities	SOP 637 - Cumulative Endpoints
SOP 612 - Working with Immunodeficient Mice	SOP 638 - Positive Reinforcement Training
SOP 613 - Working with Rodents in the Imaging Facility

SOP 701 - Macaque-Related Injury	SOP 709 - Waste Anesthetic Gas Scavenging
SOP 702 - Animal Related Injury	SOP 710 - Personal Protective Equipment Requirements
SOP 703 - Use of Viral Vectors in Neuroscience - Rodents	SOP 715 - Use of Cisplatin in Rodents
SOP 705 - Animal Containment Level 2 – Rodents	SOP 716 - Use of Doxorubicin in Rodents
SOP 706 - Use of Hazardous Agents in Rodents - General	SOP 717- Use of Tamoxifen in Rodents
SOP 708 - Use of Streptozotocin in Rodents

DC-101 Bio Security Cattle Complex	DC-606 Post-Milking Procedure
DC-121 Flushing Gutters in the Dairy Barn	DC-607 Milking Procedure
DC-131 Environmental Monitoring and Maintenance	DC-608 Milking Fresh Cows
DC-142 Infectious Agents and Zoonoses	DC-609 Milking Pail Cows
DC-206 Box Stall Sanitation	DC-610 Milking Cows with Box Stall Milker
DC-207 Tie Stall Sanitation	DC-611 Milking Staphylococcus Aureus Positive Cows
DC-208 Heifer Pen_Stall Sanitation	DC-612 Milking Antibiotic Treated Cows
DC-209 Calf Pen Sanitation	DC-613 Accidental Milking Antibiotic Treated Cows into Milking System
DC-210 Acquisition of Dairy Cattle	DC-614 Dry Treating Cows
DC-211 Loading and Transporting Cattle	DC-615 Milk Sampling
DC-212 Handling and Moving Cattle	DC-616 Milk Culturing
DC-215 Selecting Appropriate Stalls for Tethered Cattle	DC-617 California Mastitis Test (CMT)
DC-230 Environmental Enrichment Program for Dairy Cattle	DC-701 Substance Administration for Dairy Cattle
DC-301 Body Condition Scoring for Dairy Cows	DC-702 Blood Sample Collection
DC-302 Lameness Scoring for Dairy Cows	DC-712 Pre- and Post-Operative Procedures and Long-Term Care of Fistulated Cows
DC-303 Injury Scoring for Dairy Cows	DC-713 Rumen Content Collection from Fistulated Cow
DC-304 Cleanliness Assessment for Dairy Cows	DC-714 Transfaunation
DC-305 Mastitis Monitoring and Detection	DC-715 Controlled Internal Drug Release Silicone Device Insertion
DC-306 Mastitis Treatment	DC-A-1A Environmental Monitoring Log
DC-307 Extra Teat Removal Calves	DC-A-2A Cattle Transport Decision Tree
DC-311 Managing Downed Cattle	DC-A-3A Disbudding Dehorning Weight and Medication Charts
DC-312 Monitoring, Identification and Treatment of Dairy Cow Health	DC-A-3C Trainer Use Record
DC-313 Monitoring, Identification, and Treatment of Calf Health	DC-A-4A Heat Cycle, Signs and Conception rates of Dairy Cattle
DC-315 Cumulative Endpoints Dairy Cattle	DC-A-4B Signs of Heat in Dairy Cattle
DC-320 Managing Dirty Cows	DC-A-4C Factors Influencing Heat Detection in Dairy Cattle
DC-360 Outdoor Exercise Habituation of Tie-Stall Cattle	DC-A-4D Leg Band Color Legend
DC-361 Outdoor Exercise of Tie-Stall Dairy Cattle	DC-A-5A Colostrum Quality Readings
DC-400 Heat Detection and Breeding	DC-A-5B Newborn Heifer Feeding Log
DC-405 Pre-Partum Preparation of Dairy Cattle	DC-A-5C Daily Calf Monitoring Log
DC-406 Post-Partum Care of Dairy Cattle	DC-A-6A Herd Health Scoring Record
DC-407 Calving Cows-Heifers	DC-A-6B Injury Scoring Record for Dairy Cows
DC-501 Colostrum Management	DC-A-6C Lameness Scoring Record for Dairy Cows
DC-502 Milk Replacer Preparation	DC-A-6D Cleanliness Assessment Record for Dairy Cows
DC-503 Calf Identification	DC-A-6E Assessment of Rumen Fill
DC-504 Newborn Calf Care
DC-505 Calf Care Feeding
DC-506 Tube Feeding a Calf
DC-601 Milk House Pre-Milking Set Up
DC-602 Milk House Post-Milk Wash
DC-603 Bulk Tank Sanitizing Procedure
DC-604 Box Stall Milker Sanitization
DC-605 Preparing Cows for Milking

PU-101 Biosecurity Poultry Unit	PU-501 Handling Restraint
PU-202 Environmental Management Broiler	PU-520 Poultry Euthanasia
PU-203 Broiler Barn Preparation	PU-A-0 Donald McQueen Shaver Poultry Unit Floor Plan
PU-204 Sanitation Procedure Broiler Barn	PU-A-2A Broiler Temperature Management Chart
PU-205 Manure Management Broiler	PU-A-3A Should this bird be loaded - Decision tree
PU-208 Loading and Transporting Broilers	PU-A-3B Handling Guidelines for Catching Crews
PU-209 Receiving Broiler Chicks and Poults
PU-2010 Environmental Enrichment Program for Poultry

SW-2010 Environmental Enrichment Program for Swine