Standard Operating Procedures (SOPs)

Draft SOPs for reviewAnalgesia | Anesthesia | Surgery | Euthanasia | Experimental Guidelines | Animal Care Veterinary Care | Monitoring Log templateOccupational Health and Safety | Farm SOPs | SAILBody Conditioning Score | Controlled drug SOPControlled Substances Requirements | PHAC Biohazard info | ALPHA Certificate | McGill Animal Incident Report Form | Draft SOP for review by UACC | Template Crisis template |

Standard Operating Procedures (SOPs) provide a detailed description of commonly used procedures. SOPs offer investigators an alternative to writing detailed procedures in their protocol. Any deviation from the approved procedures must be clearly described and justified in the Animal Use Protocol application in Darwin. Approval of the protocol indicates approval of the deviation from the SOP for that project only.

The following Standard Operating Procedures (SOPs) were created and/or revised by the Veterinary committee and/or the Occupational Health and Safety Working Group, and approved by the Animal Policy and Welfare Oversight Committee (APWOC). They are reviewed every 3 years at most. The month and year appearing in the file name indicate when that SOP last underwent changes. Starting in 2020, the date of last review will now be specified inside the SOP even if no changes were made to it.

If you have questions or have a recommendation to make, please send an animalcare [at] (email) or talk to the Veterinarian in your area.


PDF icon420- Estrous Cycle Assessment in Rodents - July 2021



PDF icon301- Rodent Euthanasia - July 2021

PDF icon304- Avian Euthanasia - July 2021

PDF icon305- Transcardiac Perfusion - July 2021

PDF icon611- Excluded Pathogens - Rodent Facilities - July 2021

  Note that the SOP #416 Social Defeat is currently undergoing revisions again. Once done, it will be re-posted for review.













Experimental Guidelines:   

   This Monitoring Log is to be prepared by the Facility Animal Care Committee as per the approved Animal Use Protocol:  FileMonitoring log TEMPLATE - Oct 2017  

Tumor Volume

Metastatic Model Monitoring log  <- is a log

Mammary Gland Identification Mouse

Tumor Monitoring Log  <- is a log 


Animal Care:  


Veterinary Care: 



Occupational Health and Safety: 




Body Condition Score:

Body Condition Score Outline Mice (BCS)
Body Condition Score Outline For Rats (BCS)

Of interest: Article Body Condition Score for Rats

Requirements on Record Keeping, Storage and Disposal of Controlled Substances

The storage conditions and physical security for the controlled substance must meet the requirements of the "Directive on Physical Security Requirements for Controlled Substances", available on the Health Canada website.

For scientific exemptions, the required security level is Security Level - 1 and the maximum licensee holdings is $5000. Please note that each researcher must have his or her own storage.

The conditions of record keeping and disposal is outlined in the exemption letter as follows:

The researcher is required to maintain records with respect to his/her possession and use of the controlled substance in accordance with the applicable regulations. The researcher shall furnish such information respecting such controlled substances as the Minister may requires and shall permit access to the records required to be kept by these Regulations. Specifically, the researcher must keep and retain for a period of two years from the making of such record, the following information:

• the kind, date and quantity of any controlled substance purchased or received;
• the name and address of the person from whom the controlled substance was received; and particulars of the use to which the controlled substance was put.

The researcher is responsible for the destruction of any unused or expired controlled substance. The destruction must be witnessed by a member of his/her research staff who is working on the same research project as specified in the exemption, and who works under the researcher’s direction and control. The method of destruction used must alter or denature the controlled substance in such a way as to make it non-recoverable and thus make their consumption improbable or impossible. The researcher is required to keep and retain for a period of two years from the date of the making of the record, the following information:

• the name, strength per unit, and quantity of any controlled substance to be destroyed;
• the date of destruction; and
• the reason for destruction.

Immediately following the destruction, the researcher and the witness are required to sign and print their names on a joint statement indicating that they witnessed the destruction and that the controlled substance destroyed has been altered or denatured to such an extent that its consumption has been rendered impossible or improbable.

The researcher shall make such records available to the Minister or an inspector upon request.


PHAC Biohazard Information

The Public Health Agency of Canada has implemented since December 2015, new regulations pertaining to the use of Human Pathogens and Toxins. Background information can be found on their website and details on the regulation here.

In order to work with Biohazardous Materials in a laboratory setting, researchers must complete an “Application to Use Biohazardous Materials”. These applications are reviewed and approved by McGill's Environmental Health and Safety Office (not the Animal Care Committee). Approval of the Application means that the researcher is compliant with the Biosafety Programwhich includes lab inspections and training.

Useful links (these two are also available as free Apps for a smart phone or tablet as well):


Farm SOPs:

Small Animal Research Unit (SARU)

PDF iconSOP SARU_01-Storage_Room

PDF iconSOP SARU_02-Clean_Side_Cage-Washer

PDF iconSOP SARU_03-Clean_Dirty_Side_Cage_Washer

PDF iconSOP SARU_04-Sanitizing_Animal_Facility

PDF iconSOP SARU_05-Sanitizing_Operating_Room

PDF iconSOP SARU_06-Waste_Management

PDF iconSOP SARU_07-Animal_Care_Outside_Normal_Hours

PDF iconSOP SARU_08-Ventilated_Cage_Rack


Large Animal Research Unit (LARU)

PDF iconSOP LARU_01-Animal_Reception

PDF iconSOP LARU_02-Husbandry_Schedule_Swine

PDF iconSOP LARU_03-Daily_Feeding_and_Watering

PDF iconSOP LARU_04-Animal_Transport

PDF iconSOP LARU_05-Swine_Preparation_for_Surgery

PDF iconSOP LARU_06-Swine_Anesthesia_for_Surgery

PDF iconSOP LARU_07-Surgery_and_Pre-Op_Room_Cleaning

PDF iconSOP LARU_08-Controlled_Drug_Record_Keeping

PDF iconSOP LARU_09-Fire_Evacuation

PDF iconSOP laru-sop10_animal_reception_calves

PDF iconSOP laru-sop11_husbandry_schedule_calves

PDF iconSOP laru-sop12_daily_feeding_and_watering_calves

PDF iconSOP laru-sop13_calf_preparation_for_lopucalves

PDF iconSOP laru-sop14_-_rev01_anesthesia_for_lopu_calves

PDF iconSOP laru-sop15_post-lopu_care_calves

PDF iconSOP laru-sop16_fire_evacuation_plan_for_laru


Macdonald Campus Farm Cattle Complex

PDF iconSOP DC-121 Flushing Gutters in the Dairy Barn

PDF iconSOP DC-206 Box Stall Sanitation

PDF iconSOP DC-207 Tie Stall Sanitation

PDF iconSOP DC-208 Heifer Pen_Stall Sanitation

PDF iconSOP DC-209 Calf Pen Sanitation

PDF iconSOP DC-210 Acquisition of Dairy Cattle - Jan 2021

PDF iconSOP DC-212 Handling and Moving Cattle - Jan 2021

PDF iconSOP DC-301 Body Condition Scoring for Dairy Cows

PDF iconSOP DC-302 Lameness Scoring for Dairy Cows

PDF iconSOP DC-303 Injury Scoring for Dairy Cows

PDF iconSOP DC-304 Cleanliness Assessment for Dairy Cows

PDF iconSOP DC-305-2 Mastitis Monitoring and Detection

PDF iconSOP DC-306-3 Mastitis Treatment

PDF iconSOP DC-308 Dehorning and Extra Teat Removal

PDF iconSOP DC-400 Heat Detection and Breeding

PDF iconSOP DC-405-2 Post Partum Care of Dairy Cattle

PDF iconSOP DC-407 Calving Cows-Heifers

PDF iconSOP DC-501 Colostrum Management

PDF iconSOP DC-502 Milk Replacer Preparation

PDF iconSOP DC-503 Calf Identification

PDF iconSOP DC-504 Newborn Calf Care

PDF iconSOP DC-505 Calf Feeding

PDF iconSOP DC-506 Tube Feeding a Calf

PDF iconSOP DC-601 Milk House Pre-Milking Set Up

PDF iconSOP DC-602 Milk House Post-Milking Wash

PDF iconSOP DC-603 Bulk Tank Sanitizing Procedures

PDF iconSOP DC-604 Box Stall Milker Sanitization

PDF iconSOP DC-605 Preparing Cows for Milking

PDF iconSOP DC-606 Post-Milking Procedure

PDF iconSOP DC-607-3 Milking Procedure - General

PDF iconSOP DC-608-2 Milking Fresh Cows

PDF iconSOP DC-609-2 Milking Pail Cows in Tie Stalls

PDF iconSOP DC-610 Milking Pail Cows in Box Stalls

PDF iconSOP DC-611-2 Milking Staphylococcus Aureus Positive Cows

PDF iconSOP DC-612-3 Milking antibiotic treated cows

PDF iconSOP DC-613 Accidental Milking Antibiotic Treated Cows into Milking System

PDF iconSOP DC-614 Dry Treating Cows

PDF iconSOP DC-615 Milk Sampling

PDF iconSOP DC-616 Milk Culturing

PDF iconSOP DC-617 Calilfornia Mastitis Test (CMT)

PDF iconSOP DC-701-1 Substance Administration for Dairy Cattle

PDF iconSOP DC-702 Collection of Blood Sample

PDF iconSOP DC-712-1 Pre- and Post-Op Procedures and Long-Term Care of Fistulated Cows

PDF iconSOP DC-713 Rumen Content Collection from Fistulated Cow

PDF iconSOP DC-714 Transfaunation

PDF iconSOP DC-715 Controlled Internal Drug Release Silicone Device Insertion

PDF iconSOP DC-716.2 Transrectal Ultrasonography and Aspiration

PDF iconSOP DC-717 Liver Biopsy in Cattle

PDF iconSOP DC-718 Endometrial Cell Collection by Cytobrush Technique

PDF iconSOP DC-A-3A Dehorning Weight and Medication Chart

PDF iconSOP DC-A-4A.1 Heat Cycle, Signs and Conception rates of Dairy Cattle

PDF iconSOP DC-A-4B.1 Signs of Heat in dairy Cattle

PDF iconSOP DC-A-4C.1 Factors Influencing Heat Detection in Dairy Cattle

PDF iconSOP DC-A-4D-1 Leg Band Color Legend

PDF iconSOP DC-A-5A Colostrum Quality Readings

PDF iconSOP DC-A-6A Herd Health Scoring Record

PDF iconSOP DC-A-6B Injury Scoring Record for Dairy Cows

PDF iconSOP DC-A-6C Lameness Scoring Record for Dairy Cows

PDF iconSOP DC-A-6D Cleanliness Assessment Record for Dairy Cows


Donald McQueen Shaver Poultry Complex

PDF iconSOP PU-201 Biosecurity_Broiler Facility

PDF iconSOP PU-201 Biosecurity_Broiler Facility

PDF iconSOP PU-204 Sanitation Procedure_Broiler Barn

PDF iconSOP PU-205 Manure Management_Broiler

PDF iconSOP PU-208 Loading and Transporting Broilers

PDF iconSOP PU-209 Receiving Broiler Chicks and Poults

PDF iconSOP PU-301 Biosecurity_Layer Barn

PDF iconSOP PU-302 Environmental Management_Layer

PDF iconSOP PU-303 Layer Barn Preparation

PDF iconSOP PU-304 Layer Barn Sanitation Procedures

PDF iconSOP PU-305 Manure Management_Layers

PDF iconSOP PU-308 Loading and Transporting Spent Hens

PDF iconSOP PU-309 Receiving Layer Hens

PDF iconSOP PU-404 Egg Collection Room 100

PDF iconSOP PU-405 Egg Collection Room 200

PDF iconSOP PU-A-2A Broiler Temperature Management Chart_Draft

PDF iconSOP PU-A-3A Should this bird be loaded_Decision tree

PDF iconSOP PU-A-3B Handling Guidelines for Catching Crews


Small Animal Imaging Labs (SAIL)

PDF iconSAIL-SOP-01-Rodent Transportation Procedure

PDF iconSAIL-SOP-02-Animal Management and Housing at the SAIL Facility

PDF iconSAIL-SOP-03- Working with CL2 Animals

PDF iconSAIL-MRI-SOP-01-Pre-scan Procedures

PDF iconSAIL-MRI-SOP-02-Anesthesia Induction and Maintenance

PDF iconSAIL-MRI-SOP-03-In-Scan Subject Health Monitoring

PDF iconSAIL-MRI-SOP-04-Post-Scan Recovery and Follow-up

PDF iconSAIL-MRI-SOP-05-Cleaning and Decontamination

PDF iconSAIL-MRI-SOP-06-Daily Maintenance and Cleaning

PDF iconSAIL-MRI-SOP-07-Weekly Maintenance and Cleaning

PDF iconSAIL-PET-SOP-01-Radioligand Handling

PDF iconSAIL-PET-SOP-02-Animal and Scanner Preparation

PDF iconSAIL-PET-SOP-03-Anesthesia Induction and Maintenance

PDF iconSAIL-PET-SOP-04-Scanning and Monitoring

PDF iconSAIL-PET-SOP-05- Post-scan Recovery and Follow-up

PDF iconSAIL-PET-SOP-06-Cleaning and Decontamination

PDF iconSAIL-PET-SOP-08-Weekly Maintenance and Cleaning

PDF iconSAIL-PET-SOP-09-Radioactive Cages Management

PDF iconSAIL-Xtreme-SOP-01-Animal and Imager Preparation

PDF iconSAIL-Xtreme-SOP-02-Anesthesia Induction and Maintenance

PDF iconSAIL-Xtreme-SOP-03-Animal Recovery and Follow-up

PDF iconSAIL-Xtreme-SOP-04-Cleaning and Decontamination

PDF iconSAIL-Xtreme-SOP-05-Daily Maintenance and Cleaning


McGill University Animal Incident Report Form: 

FileMcGill Animal Care Incident Report Form - 2013


Template for a Crisis Management and Communication Plan SOP:

FileSOP TEMPLATE for Crisis Management Plan - April 2021



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