Standard Operating Procedures (SOPs)

Draft SOPs for reviewAnalgesia | Anesthesia | Surgery | Euthanasia | Experimental Guidelines | Animal Care Veterinary Care | Monitoring Log templateOccupational Health and Safety | SAILBody Conditioning Score | Farm SOPs| Controlled drug SOPControlled Substances Requirements | PHAC Biohazard info | ALPHA Certificate | McGill Animal Incident Report Form | Draft SOP for review by APWOC (formerly UACC) | Template Crisis template |

Standard Operating Procedures (SOPs) provide a detailed description of commonly used procedures. SOPs offer investigators an alternative to writing detailed procedures in their protocol. Any deviation from the approved procedures must be clearly described and justified in the Animal Use Protocol application in Darwin. Approval of the protocol indicates approval of the deviation from the SOP for that project only.

The following Standard Operating Procedures (SOPs) were created and/or revised by the Veterinary Committee and/or the Occupational Health and Safety Working Group, and approved by the Animal Policy and Welfare Oversight Committee (APWOC). They are reviewed every 3 years at most. The month and year appearing in the file name indicate when that SOP last underwent changes. Starting in 2020, the date of last review will now be specified inside the SOP even if no changes were made to it.

If you have questions or have a recommendation to make, please send an animalcare [at] (email) or talk to the Veterinarian in your area.

DRAFT SOP : None for review

Note that the SOP #416 Social Defeat is currently undergoing revisions again. Once done, it will be re-posted for review.













Experimental Guidelines:   

   This Monitoring Log is to be prepared by the Facility Animal Care Committee as per the approved Animal Use Protocol:  FileMonitoring log TEMPLATE - Oct 2017  

Tumor Volume

Metastatic Model Monitoring log  <- is a log

Mammary Gland Identification Mouse

Tumor Monitoring Log  <- is a log 


Animal Care:  


Veterinary Care: 



Occupational Health and Safety: 




Body Condition Score:

Body Condition Score Outline Mice (BCS)
Body Condition Score Outline For Rats (BCS)

Of interest: Article Body Condition Score for Rats



Dairy Facility SOPs:

PDF iconSOP DC-121 Flushing Gutters in the Dairy Barn

PDF iconSOP DC-206 Box Stall Sanitation

PDF iconSOP DC-207 Tie Stall Sanitation

PDF iconSOP DC-208 Heifer Pen_Stall Sanitation

PDF iconSOP DC-209 Calf Pen Sanitation

PDF iconSOP DC-210 Acquisition of Dairy Cattle

PDF iconSOP DC-212 Handling and Moving Cattle

PDF iconSOP DC-301 Body Condition Scoring for Dairy Cows

PDF iconSOP DC-302 Lameness Scoring for Dairy Cows

PDF iconSOP DC-303 Injury Scoring for Dairy Cows

PDF iconSOP DC-304 Cleanliness Assessment for Dairy Cows

PDF iconSOP DC-305.2 Mastitiis Monitoriing and Detection

PDF iconSOP DC-306.3 Mastitis Treatment

PDF iconSOP DC-308 Dehorning and Extra Teat Removal

PDF iconSOP DC-400 Heat Detection and Breeding

PDF iconSOP DC-405.2 Post Partum Care of Dairy Cattle

PDF iconSOP DC-407 Calving Cows-Heifers

PDF iconSOP DC-501 Colostrum Management

PDF iconSOP DC-502 Milk Replacer Preparation

PDF iconSOP DC-503 Calf Identification

PDF iconSOP DC-504 Newborn Calf Care

PDF iconSOP DC-505 Calf Feeding

PDF iconSOP DC-506 Tube Feeding a Calf

PDF iconSOP DC-601 Milk House Pre-Milking Set Up

PDF iconSOP DC-602 Milk House Post-Milking Wash

PDF iconSOP DC-603 Bulk Tank Sanitizing Procedures

PDF iconSOP DC-604 Box Stall Milker Sanitization

PDF iconSOP DC-605.2 Preparing Cows for Milking

PDF iconSOP DC-606.2 Post-Milking Procedure

PDF iconSOP DC-607.3 Milking Procedure - General

PDF iconSOP DC-608.2 Milking Fresh Cows

PDF iconSOP DC-609.2 Milking Pail Cows in Tie Stalls

PDF iconSOP DC-610 Milking Pail Cows in Box Stalls

PDF iconSOP DC-611.2 Milking Staphylococcus Aureus Positive Cows

PDF iconSOP DC-612.3 Milking antibiotic treated cows

PDF iconSOP DC-613 Accidental Milking Antibiotic Treated Cows into Milking System

PDF iconSOP DC-614 Dry Treating Cows

PDF iconSOP DC-615 Milk Sampling

PDF iconSOP DC-616 Milk Culturing

PDF iconSOP DC-617 Calilfornia Mastitis Test (CMT)

PDF iconSOP DC-701.1 Substance Administration for Dairy Cattle

PDF iconSOP DC-702 Collection of Blood Sample

PDF iconSOP DC-712.1 Pre- and Post-Operative Procedures and Long-Term Care of Fistulated Cows

PDF iconSOP DC-713 Rumen Content Collection from Fistulated Cow

PDF iconSOP DC-714 Transfaunation

PDF iconSOP DC-715 Controlled Internal Drug Release Silicone Device Insertion

PDF iconSOP DC-716.2 Transrectal Ultrasonography and Aspiration

PDF iconSOP DC-717 Liver Biopsy in Cattle

PDF iconSOP DC-718.2 Endometrial Cell Collection by Cytobrush Technique

PDF iconDC-A-3A Dehorning Weight and Medication Chart

PDF iconDC-A-4A.1 Heat Cycle, Signs and Conception rates of Dairy Cattle

PDF iconDC-A-4B.1 Signs of Heat in dairy Cattle

PDF iconDC-A-4C.1 Factors Influencing Heat Detection in Dairy Cattle

PDF iconDC-A-4D.1 Leg Band Color Legend

PDF iconDC-A-5A Colostrum Quality Readings

PDF iconDC-A-6A Herd Health Scoring Record

PDF iconDC-A-6B Injury Scoring Record for Dairy Cows

PDF iconDC-A-6C Lameness Scoring Record for Dairy Cows

PDF iconDC-A-6D Cleanliness Assessment Record for Dairy Cows

Poultry Facility SOPs:

PDF iconSOP PU-201 Biosecurity_Broiler Facility

PDF iconSOP PU-203 Broiler Barn Preparation

PDF iconSOP PU-204 Sanitation Procedure_Broiler Barn

PDF iconSOP PU-205 Manure Management_Broiler

PDF iconSOP PU-208 Loading and Transporting Broilers

PDF iconSOP PU-209 Receiving Broiler Chicks and Poults

PDF iconPU-301 Biosecurity_Layer Barn

PDF iconSOP PU-302 Environmental Management_Layer

PDF iconSOP PU-303 Layer Barn Preparation

PDF iconSOP PU-304 Layer Barn Sanitation Procedures

PDF iconSOP PU-305 Manure Management_Layers

PDF iconSOP PU-308 Loading and Transporting Spent Hens

PDF iconSOP PU-309 Receiving Layer Hens

PDF iconSOP PU-404 Egg Collection Room 100

PDF iconSOP PU-405 Egg Collection Room 200

PDF iconPU-A-2A Broiler Temperature Management Chart_Draft

PDF iconPU-A-3A Should this bird be loaded_Decision tree

PDF iconPU-A-3B Handling Guidelines for Catching Crews


Requirements on Record Keeping, Storage and Disposal of Controlled Substances

Refer to the McGill Environmental Health and Safety Office's SOP 401 on Cannabis and Controlled Substances.

Research Institutes of affiliated hospitals should contact their local Safety Office or EHS Officer.

For McGill researchers and instructors:

• All Principal Investigators (PIs) intending to use controlled substances (and cannabis) need to contact the domenic.dulisse [at] (EHS Cannabis & Controlled Substances Officer) for notification and exemption processing. The PI's information is added to EHS' records and follow ups are performed. All research and teaching involving animals MUST have an approved Animal Use Protocol before being considered for an exemption.
• The EHS' MyLab software migration will allow for this information to be kept and tracked within MyLab. Permits will be issued to PIs using cannabis and controlled substances.
• Training for cannabis and controlled substance will be mandatory for all users. They will be granted a permit contingent on successful completion of the training which is every 2 years.
• An inspection checklist has been created specifically for cannabis and controlled substances in labs. PIs must follow Health Canada’s directive on physical security, while McGill veterinarians do not (they are responsible for the safe keeping of their drugs and reporting to Health Canada).
• A Cannabis compliance committee is being set up with the Director of EHS and will allow for better decision making and department awareness. This will be done for controlled substances in the near future.
• Controlled drug suppliers have been added to the McGill Market Place (MMP) and follow the same rules as radiation materials. Note that all orders must be approved by the cannabis and controlled substance officer.
• A revised SOP #401 for controlled substances is now posted on the EHS site. There is more information on EHS' website for the Cannabis and Controlled Substance Compliance Program (CCSCP) , Environmental Health and Safety, McGill University.


PHAC Biohazard Information

The Public Health Agency of Canada has implemented since December 2015, new regulations pertaining to the use of Human Pathogens and Toxins. Background information can be found on their website and details on the regulation here.

In order to work with Biohazardous Materials in a laboratory setting, researchers must complete an “Application to Use Biohazardous Materials”. These applications are reviewed and approved by McGill's Environmental Health and Safety Office (not the Animal Care Committee). Approval of the Application means that the researcher is compliant with the Biosafety Programwhich includes lab inspections and training.

Useful links (these two are also available as free Apps for a smart phone or tablet as well):




Small Animal Imaging Labs (SAIL)

PDF iconSAIL-SOP-01-Rodent Transportation Procedure

PDF iconSAIL-SOP-02-Animal Management and Housing at the SAIL Facility

PDF iconSAIL-SOP-03- Working with CL2 Animals

PDF iconSAIL-MRI-SOP-01-Pre-scan Procedures

PDF iconSAIL-MRI-SOP-02-Anesthesia Induction and Maintenance

PDF iconSAIL-MRI-SOP-03-In-Scan Subject Health Monitoring

PDF iconSAIL-MRI-SOP-04-Post-Scan Recovery and Follow-up

PDF iconSAIL-MRI-SOP-05-Cleaning and Decontamination

PDF iconSAIL-MRI-SOP-06-Daily Maintenance and Cleaning

PDF iconSAIL-MRI-SOP-07-Weekly Maintenance and Cleaning

PDF iconSAIL-PET-SOP-01-Radioligand Handling

PDF iconSAIL-PET-SOP-02-Animal and Scanner Preparation

PDF iconSAIL-PET-SOP-03-Anesthesia Induction and Maintenance

PDF iconSAIL-PET-SOP-04-Scanning and Monitoring

PDF iconSAIL-PET-SOP-05- Post-scan Recovery and Follow-up

PDF iconSAIL-PET-SOP-06-Cleaning and Decontamination

PDF iconSAIL-PET-SOP-08-Weekly Maintenance and Cleaning

PDF iconSAIL-PET-SOP-09-Radioactive Cages Management

PDF iconSAIL-Xtreme-SOP-01-Animal and Imager Preparation

PDF iconSAIL-Xtreme-SOP-02-Anesthesia Induction and Maintenance

PDF iconSAIL-Xtreme-SOP-03-Animal Recovery and Follow-up

PDF iconSAIL-Xtreme-SOP-04-Cleaning and Decontamination

PDF iconSAIL-Xtreme-SOP-05-Daily Maintenance and Cleaning


McGill University Animal Incident Report Form: 

FileMcGill Animal Care Incident Report Form - 2013


Template for a Crisis Management and Communication Plan SOP:

FileSOP TEMPLATE for Crisis Management Plan - April 2021



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