Shafaat A. Rabbani, M.D.
Montreal, Quebec H4A 3J1
shafaat.rabbani [at] mcgill.ca
Dr. Shafaat Rabbani is a Professor of Medicine at McGill University Montreal, Canada. He is also associate member of Departments of Physiology at Oncology at McGill University Health Centre. Dr. Rabbani received his medical education at King Edward Medical College Lahore Pakistan. His research led to the characterization of Parathyroid Hormone Related Peptide as the main pathogenetic factor responsible of hypercalcemia of malignancy. He developed several syngenic and xenograft models of breast and prostate cancer which mimicked the ability of these malignancies to metastasize to the skeleton. Pioneering work carried out in his laboratory led to identification Urokinase as a mitogen for cells of the osteoblast phenotype and a key protease involved in tumor invasion, growth and metastasis. His group was the first to demonstrate the role of DNA methylation as a key molecular mechanism regulating urokinase gene transcription. Dr. Rabbani serves as a reviewer for several high impact journals and on several study sections in Canada and United States. Dr. Rabbani has issued and filed patents in his area of expertise which has continued to be the role of growth factors and proteases in skeletal metastasis. Over the years he has served as a consultant and member if the scientific advisory board to of number biotechnology and pharmaceutical companies in Canada and United States.
Epigenetics, DNA methylation, breast Cancer, prostate Cancer, urokinase, osteoporosis, DNA methylation, parathyroid hormone related peptide
Research or Clinical Activities
Using blood from patients with breast cancer and osteoporosis we are determining the DNA methylation landscape of these patients. The blood based “DNA methylation signatures” will be customized as noninvasive exquisite biomarkers that will be introduced into clinical practice and change the way we predict, diagnose stratify for therapy and follow up the progression of the disease.
We are evaluating novel therapeutic and preventive approach to breast cancer metastasis based on blocking DNA demethylation processes using well established xenograft and transgenic models of breast cancer. Collectively the results from these studies will provide a molecular mechanistic basis for both prevention and treatment of breast cancer which will be translated into human clinical trials.
Our group is developing novel diagnostic and therapeutic strategies to block uPA production and its interaction with cell surface uPAR to block tumor growth and metastasis. Using our well- established models of breast and prostate cancer; we are examining the efficacy of various therapeutic strategies to block tumor progression. Additionally determination of the methylation of uPA and PAI-1 is being developed as a reliable diagnostic and prognostic marker in cancer.
Selected Recent Publications
1. Shukeir N, Stefanska B, Parashar S, Chik F, Arakelian A, Szyf M, Rabbani SA. Pharmacological methyl group donors block skeletal metastasis in vitro and in vivo. Br J Pharmacol. 2015; 172(11):2769-81.
2. Parashar S, Cheishvili D, Arakelian A, Hussain Z, Tanvir I, Khan HA, Szyf M, Rabbani SA.S-adenosylmethionine blocks osteosarcoma cells proliferation and invasion in vitro and tumor metastasis in vivo: therapeutic and diagnostic clinical applications. Cancer Med. 2015 ;4(5):732-44.
3. Cheishvili D, Chik F, Li CC, Bhattacharya B, Suderman M, Arakelian A, Hallett M, Rabbani SA, Szyf M. Synergistic effects of combined DNA methyltransferase inhibition and MBD2 depletion on breast cancer cells; MBD2 depletion blocks 5-aza-2'-deoxycytidine-triggered invasiveness. Carcinogenesis. 2014;35(11):2436-46.
4. Stefanska B, Cheishvili D, Suderman M, Arakelian A, Huang J, Hallett M, Han ZG, Al-Mahtab M, Akbar SM, Khan WA, Raqib R, Tanvir I, Khan HA, Rabbani SA, Szyf M. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin. Cancer Res. 2014; 20(12):3118-32.
5. Rabbani SA, Arakelian A, Farookhi R. LRP5 knockdown: effect on prostate cancer invasion growth and skeletal metastasis in vitro and in vivo. Cancer Med. 2013;2(5):625-35.