Stéphane A. Laporte
Stéphane A. Laporte, Ph.D.
Associate Professor, Department of Medicine
stephane [dot] laporte [at] mcgill [dot] ca
Dr. Laporte received his undergraduate degree in Biology (Major Biotechnology), and M.Sc. and Ph.D. degrees in Pharmacology at the Université de Sherbrooke, Quebec, Canada. He trained as a postdoctoral fellow in the group Dr. Marc G. Caron, at Duke University, USA, before joining McGill in 2001.
G protein-coupled receptors, arrestins, signaling, trafficking, confocal, BRET, FRET, cardiovascular diseases, and pre-term birth
Research or Clinical Activities
Our laboratory focuses on understanding the molecular and cellular mechanisms regulating G protein-coupled receptors (GPCRs). We are interested in ligand-directed signaling, and the development of novel molecules with allosteric, biased signaling modes of action, in particular for the prostanglandin PG2α (FP) receptor. Using innovative imaging and biophysical approaches we also study the dynamics of protein complexes involved in receptor trafficking and signaling for different GPCRs, in particular for the angiotensin II type 1 and Bradykinin B2 receptors. Our laboratory is involved in developing novel fluorescently tagged biosensors for studying, in live cells, GPCR signaling and trafficking, with the goal of identifying small molecules regulator of these responses. Our research program aims at providing new insights into the molecular details of GPCR signaling and trafficking, with the goal of identifying new means to improve hormone and drug efficacy, especially in cardiovascular diseases, inflammation, pre-term birth and cancer.
Selected Recent Publications
Zimmerman B, Beautrait A, Aguila B, Charles R, Claing A, Escher E, Bouvier M and Laporte SA. Differential βarrestin-dependent conformation signalling and cellular responses revealed by angiotensin analogs. Sci Signal. (2012) Apr; 5(221): ra33. PMID: 22534132
Goupil E , Wiseheart V , Khoury E , Zimmerman B, Jaffal S, Hébert TE and Laporte SA. Biasing the prostaglandin F2alpha receptor response toward EGFR-dependent transactivation. Mol Endocrinol. (2012) Jul; 26(7):1189-202. PMID:22638073
Aguila B, Simaan M and Laporte SA. Study of G Protein-Coupled Receptor/β-arrestin Interactions Within Endosomes Using FRAP. Methods Mol Biol. (2011) 756(6): 371-380. PMID:21870240
Goupil E, Tassy D, Bourguet C, Quiniou C, Wisehart V, Pétrin D, Le Gouill C, Devost D, Zingg HH, Bouvier M, Saragovi HU, Chemtob S, Lubell WD, Claing A, Hébert TE, Laporte SA. A novel biased allosteric compound inhibitor of parturition selectively impedes the prostaglandin F2alpha-mediated Rho/ROCK signaling pathway. J Biol Chem. (2010) 285(33):25624-36. PMID:20551320
Gousseva V, Simaan M, Laporte SA, and Swain PS. Inferring the lifetime of endosomal complexes by fluorescence recovery after photobleaching. Biophys J. (2008) 15;92(2): 679-87. PMID: 17827242