Agilent 1260 Infinity II GPC

Contact: violeta.toader [at] (Violeta Toader) - Location: OM 21

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Size Exclusion Chromatography is a widely used techniques for detection of average molecular weights and their distribution of a polymer sample.

A small volume of a diluted polymer solution in an appropriate solvent (mobile phase or eluent) is passed through a column packed with porous “gel” beads (stationary phase). Separation occurs through the partitioning of polymer molecules into the pore structure of the beads due to chain hydrodynamic volume differences with large molecules eluting first. A graph is generated based on elution (or retention time) versus the signal from a specific detector. Using standards of determined MW and narrow distribution, a column calibration curve is constructed that correlates mathematically standards MW with their retention time the retention time of the analyzed sample is then used to detect its average molecular weights and distribution.

Features at a glance

This robust system is used for analyses of aqueous polymer solution within 3 -10 pH range.

The system is equipped with autosampler, a column oven (up to 85°C), and compact Refractive Index and dual Light Scattering Detectors (16 and 90 degree) in one unit.

Column calibration available with pullulan (only in the range 5 000 to 800 000 g/mol) and PEO standards.

Choice of column and eluent must be discussed with staff beforehand.

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