Molecular Expressions (FSU), Microscopy Resources Center (Olympus), Education in Microscopy and Digital Imaging (Zeiss) and MicroscopyU (Nikon). Four quite exhaustive sites (very similar in content and organization), covering everything from basic microscopy to more specialized subjects.
ScienceLab (Leica), less organized but searchable collection of articles, webinars and tutorials on a variety of subjects.
BitesizeBio, Microscopy and Imaging, short articles and company-sponsored webinars covering a variety of different microscopy-related subjects.
iBiology Microscopy Course, lecture videos from basic microscopy to specialised applications.
Some introductory reviews:
Fluorescence microscopy (Nature Methods)
Optical sectioning microscopy (Nature Methods)
Second harmonic imaging microscopy...(Nature Biotechnology)
Fluorescence lifetime imaging-techniques and applications (Journal of Microscopy)
Seeing is believing? A beginners' guide to practical pitfalls in image acquisition (Journal of Cell Biology)
Accuracy and precision in quantitative fluorescence microscopy (Journal of Cell Biology)
Interactive databases of fluorophore excitation/ emission curves and filters:
Spectraviewer (Life technologies), covers mainly their offering of fluorophores, allows to customize the filter specifications.
Curvomatic (Chroma) and Searchlight (Semrock), both are filter manufacturers and only list their filter-sets. Their databases contain many fluorphores that are not listed in the Spectraviewer.
Handbook on filters for fluorescence microscopy (Chroma), a compilation of filter design and terminology.
2-photon excitation spectra (drbio.cornell)
Two-photon absorption properties of fluorescent proteins (Nature methods)
Fluorescent dyes and techniques:
Fluorescence and IHC reference images:
Primary antibody search:
Information about Leica microscopes
Leica objectives, explains the objective nomenclature and objective specifications.
ScienceLab, searchable Leica microscopy resources site with articles, webinars, tutorials.
Leica AF Lite, free download, allows to view .lif images and save them in open image formats.
PMT and HyD detectors, explains how Hybrid detectors offer higher sensitivity than standard PMT detectors.
Operetta Application Notes (PerkinElmer), High Content Screening page with examples for cellular assays and analysis methods.
High Content Screening Assay Development Guidelines, introduction to High Content Imaging/ Screening and best practices for assay development.
Tips for HCS-assays (Enzo)
Tips for cell-based assays (Enzo)
Assay quality: the Z’-Factor (Journal of Biomolecular Screening), commonly used simple statistical method to evaluate HTS/ HCS assays.
Real Time PCR Handbook (Life Technologies)
Guide to performing Relative quantitation of gene expression (Applied Biosystems)
The MIQE Guidelines-Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Pitfalls of Quantitative Real-Time Reverse-Transcription PCR (Journal of biomolecular techniques)
DNA Methylation Analysis Protocol (Nature Protocols)
PCR optimization for pyrosequencing (Biotage)
Guidelines for allele quantification (Standford University)
Pyrosequencing troubleshooting guidelines (Standford University)
GeneSpring Support: Video Tutorials, Webinars, Demo Datasets
Agilent earray: Catalog and Custom Microarray designs
GeneSpring manual (Agilent)
CGH interactive analysis (Agilent)
Methylation analysis (Agilent)
ChIP interactive analysis (Agilent)
in situ RNA detection and Immunohistochemistry
Automation and Transfection/ Injection
Liquid handling resources Janus (PerkinElmer)
Biolistic transfection, Helios gene gun (Bio Rad)