Nikon CSUX-1 Spinning Disk Confocal (Biosafety Level 2)
Applications:
- Intravital Imaging
- 4D Confocal
- Fast Kinetics
- Widefield Fluorescence
- Brightfield
- Tilling
Excitation:
- Four laser lines for confocal imaging: 405nm, 488nm, 561nm, 640nm.
- Four LED lines for widefield fluorescence: 385nm, 488nm, 561nm, 621nm.
- Brightfield illumination.
Emission:
- Four single chanel emission filters: 455/50nm, 525/36nm, 605/52nm, 705/72nm.
- One quad chanels filter for high speed acquisition: DAPI/FITC/TRITC/CY5.
Detection:
- Confocal: Photometrics Prime BSI Express sCMOS Camera. 2048x2048, 95fps@11-bits/43fps@16-bits.
- Widefield and Brigthfield: Nikon Digital Sight 10, Color and Monochrome mode, 25 mm FOV, 23.9 megapixel.
Objectives:
-
10X/0.45 air, 20X/0.75 air, 16X/0.8 water dipping, 20X/1.0 water, 40X/0.8 water dipping, 60X/1.42 oil.
Located in a biosafety level 2 room that include a BSC.
Image processing and analysis: Nikon imaging software (NIS) workstation and Bitplane Imaris 9.1.2.
LEICA SP8 Laser Scanning Confocal and Multiphoton
Applications: 4D Confocal and Multiphoton Imaging, Spectral imaging, FLIM, FRET, FRAP modules, SHG
Excitation:
- Four solid state laser lines: 448 nm, 488 nm, 552 nm,
638 nm
- One tunable IR two-photons laser: 680-1080 nm
Detection:
- Two internal HyDs (high sensitivity detectors)
- Two internal PMTs
- One transmitted light PMT
- Two reflected light HyD
- One brightfield detector (Dodt gradient)
Objectives: 4x to 63x (including air, oil and multi-immersion)
Environmental control: CO2, temperature, humidity.
Image processing and analysis: Leica LAS X and Bitplane Imaris 9.1.2
