There has been significant progress over the last decade in understanding the molecular basis by which sensory neurons transduce and subsequently transmit noxious (ie. tissue damaging) stimuli giving rise to the sensation of pain. Over this same period we have recognized that mutations in such ion channels (many of which are selectively expressed in sensory neurons) can result in inherited pain disorders in humans.
Do you ever wonder what is in the air you breathe? You should- given that you take around 20,000 breaths per day. Did you also know that cigarette smoke contributes to the toxicity of common air contaminants and remains the largest cause of preventable death worldwide? Despite knowledge about the deleterious effects of tobacco, we are seeing a surge in the use of emerging tobacco-based products such as e-cigarettes.
In the developing nervous system, an enormous number and diversity of neurons are precisely organized into neural circuits. How can such a vast set of neural connections be wired using limited cues encoded in our genomes? To tackle this problem, we are studying a family of neuronal receptors with an extraordinary potential for conferring cell-surface diversity and wiring specificity.
Fructose is a simple sugar found in fruit and honey, but it is also used as sweeteners via added sugars, syrups, or high fructose corn syrup in processed foods or beverages. This is a concern because excessive fructose intake is linked to obesity and its comorbid diseases like diabetes and cardiovascular disease. We show that mice consuming a high fructose diet eat more and gain more weight and body fat than mice consuming chow or a high glucose diet.
Seminar - Regulation of calcium channel activity in cardiomyocytes : what is the role of auxiliary subunits?
The normal heartbeat is conditioned by transient increases in the intracellular free Ca2+ concentration. Ca2+ influx in cardiomyocytes is regulated by the activity of the heteromeric L-type voltage-activated CaV1.2 channel. A complex network of interactions between the different proteins forming the ion channel determines the total Ca2+ influx.
Seminar: Single-cell transcriptomic analysis of acute myeloid leukemia and immune checkpoint blockade
Acute myeloid leukemia (AML) is an aggressive cancer of the myeloid lineage of the blood system. Prior work has shown that a rare subset of leukemic stem cells (LSCs) is able to propagate the disease. LSCs are presently only defined functionally as able to engraft by xenotransplantation in immunocompromised mice. No universal markers for LSCs are currently known, therefore previous efforts to study LSCs have relied on enriching for them by cell sorting with non-specific markers.
Mutation or disruption of the SHANK3 (SH3 domain and ankyrin repeat) gene at the 22q13.3 locus represents a highly penetrant, monogenic risk-factor for Autism Spectrum Disorder (ASD) and is a leading cause of Phelan–McDermid Syndrome (PMS). Recent advances in gene editing enabled the creation of genetically engineered non-human primate (NHP) models of brain disorders.
This event is part of the Department of Physiology Friday Seminar Series and is co-sponsored by the Alan Edwards Centre for Research on Pain (AECRP)
The human naive T-cell receptor (TCR) repertoire is extremely diverse and accurately estimating its distribution is challenging. We address this challenge by combining a quantitative sequencing protocol of TCRA and TCRB sequences with computational modeling. We observed the vast majority of TCR chains only once in our samples, confirming the enormous diversity of the naive repertoire.
More details coming soon!
In vertebrates, the response elicited by invading pathogens involves a sophisticated interplay of cells that constitute the innate and adaptive immune systems. This concerted response – precise in its quality, magnitude and duration – is fundamental for the elimination or control of infectious agents and the repair of the affected tissue/organ. Imprecision in the response can have serious deleterious consequences including damage to host tissue or failure to eliminate the pathogen.
Machine learning research has had and will continue to have a transformative impact in healthcare and medicine. This talk will discuss a few research directions which can have a transformative impact as well as their challenges which we are working on. I will discuss chest xray diagnostic tools and challenges with training them. I will discuss representation learning for electrocardiogram (ECG) data and the release of a large public dataset.
Under the mentorship of Dr Rejean Couture, Dr Talbot obtained is PhD in Physiology from the Université de Montreal (2012). His thesis focused on the role of CNS immune cells in priming diabetic pain neuropathy. Next, he joined Dr. Clifford Woolf’s lab at the Neurobiology center of Harvard Medical School to study the crosstalk between pain neurons and adaptive immune cells in the context of allergic inflammation. Since 2017, Dr Talbot joined the UdeM pharmacology and physiology dept.
More details coming soon!