Resources

The Department of Pharmacology & Therapeutics at McGill University maintains several key resources for use by faculty and students for their research.

Confocal Facility

The Department hosts a confocal microscopy/image analysis facility. This facility includes a multi-photon Zeiss 510 laser scanning microscope which is available to the scientific community on a fee basis.

Contact info for Confocal Facility:

Manon St.Louis
McIntyre Building
Room: 1242
manon.st_louis [at] mcgill.ca
514-398-8841

The facility also has two automated image analysis systems (M4 MCID system and MCID Elite system), both coupled to Olympus microscopes. The MCID Elite system is coupled to an Olympus BX51 microscope with a motorized stage and focus and possesses 3-D reconstruction, stereology and tiled field mapping modules.

EM Services

The department’s Electron Microscopy facility features a fully equipped electron microscope (FEI CM 120) with a video GATAN digital camera system. The facility also includes two Reichert Ultratome ultramicrotomes. This facility is specialized in classical transmission electron microscopy (TEM) for biological specimens, which is what most researchers in the fields of Pharmacology, Biochemistry, Molecular Biology, Physiology and Cancer Research need. Our Facility allows easy access to EM services to users without training in the technique thanks to the availability of a full time highly experienced technician. This is particularly important for some investigators who lack expertise in EM, but who are planning to do studies containing an EM component to be published in high impact journals.  Articles from labs using our facility were recently published in top journals such as Nature Medicine (Joyal et al., Vol. 20:1165-11732014), Nature Communications (Vincent et al., 7:10604, 2016), Cell Discovery (Bhosle et al., vol 2:16017, 2016) and Cell Reports (Petitjean et al., vol. 13:1246-1257, 2015).

PDF icon Recent Publications from our Electron Microscopy Facility

PDF icon High Quality Micrographs from our Electron Microscopy Facility

Contact info for EM Facility:

Johanne Ouellette
McIntyre Building
Room: 119
514-398-3606
johanne.ouellette [at] mcgill.ca

Imaging and Molecular Biology Platform

The Platform is part of the Green Chemistry CFI initiative and a centralized resource for microscopy and molecular biology equipment. The platform provides instruments, training and assistance to internal and external researchers. Microscopy services range from confocal/multiphoton and high throughput to in vivo imaging and analysis. Histological equipment and automated slide staining is also available. Molecular applications cover quantitative and qualitative sample analysis, gene expression characterization and genomics. Please click here for more information.

Contact info for the Imaging and Molecular Biology Platform:

McIntyre Medical Building - Room 1319
Phone:  514-398-4455, extension 09036
impb [at] mcgill.ca

SPR-MS Facility

The Surface Plasmon Resonance - Mass Spectrometry (SPR-MS) Facility includes:

  • Bruker UltrafleXtreme system for MALDI-MS of peptides/proteins
  • Bruker ImagePrep system to prepare tissue sections for MALDI imaging
  • Bruker Impact II system for complementary ESI-MS of peptides/proteins
  • BIACORE 3000 system for label-free, real-time protein interaction analysis

PDF icon recent_spr-ms_facility_publications.pdf

Contact us to discuss your projects:
Mark Hancock, Ph.D.
McGill University
McIntyre Medical Sciences Bldg
mark.hancock [at] mcgill.ca
514-398-1350

Other resources

In addition, the department maintains a phosphorimaging system (Molecular Dynamics) and other standard equipment for common use.