Recent Publications
Kahn RA, Virk HS, McPherson PS. (2023) Heed a decade of calls for antibody validation. Nature. 2023 Aug;620(7974):492. doi: 10.1038/d41586-023-02566-w.
Kumar R, Francis V, Ioannou MS, Aguila A, Khan M, Banks E, Kulasekaran G, McPherson P.S. (2023) DENND2B activates Rab35 at the intercellular bridge, regulating cytokinetic abscission and tetraploidy. Cell Rep. 2023 Jul 25;42(7):112795. doi: 10.1016/j.celrep.2023.112795
Ayoubi R, Ryan J, Biddle MS, Alshafie W, Fotouhi M, Bolivar SG, Moleon VR, Eckmann P, Worrall D, McDowell I, Southern K, Reintsch W, Durcan TM, Brown CM, Bandrowski A, Virk HS, Edwards AM, McPherson PS, Laflamme C. (2023) Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications. bioRxiv. 2023 Aug 17:2023.06.01.543292. doi: 10.1101/2023.06.01.543292.
Bayati A, Luo W, Del Cid-Pellitero E, Fon EA, Durcan TM, McPherson PS. (2023) STAR Protoc. Visualization of α-synuclein trafficking via nanogold labeling and electron microscopy. 2023 Feb 23;4(1):102113. doi: 10.1016/j.xpro.2023.102113.
Drewry DH, Potjewyd FM, Bayati A, Smith JL, Dickmander RJ, Howell S, Taft-Benz S, Min SM, Hossain MA, Heise M, McPherson PS, Moorman NJ, Axtman AD. Identification and Utilization of a Chemical Probe to Interrogate the Roles of PIKfyve in the Lifecycle of β-Coronaviruses. J Med Chem. 2022 Oct 13;65(19):12860-12882. doi: 10.1021/acs.jmedchem.2c00697.
Francis V, Alshafie W, Kumar R, Girard M, Brais B, McPherson PS. (2022) The ARSACS disease protein sacsin controls lysosomal positioning and reformation by regulating microtubule dynamics. J Biol Chem. 2022 Sep;298(9):102320. doi: 10.1016/j.jbc.2022.102320.
Bayati A, Banks E, Han C, Luo W, Reintsch WE, Zorca CE, Shlaifer I, Del Cid Pellitero E, Vanderperre B, McBride HM, Fon EA, Durcan TM, McPherson P.S. (2022) Rapid macropinocytic transfer of α-synuclein to lysosomes. Cell Rep. 2022 Jul 19;40(3):111102. doi: 10.1016/j.celrep.2022.111102.
Kumar R, Francis V, Kulasekaran G, Khan M, Armstrong GAB, McPherson P.S. (2022) A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis. Sci Adv. 2022 Feb 25;8(8):eabk3088. doi: 10.1126/sciadv.abk3088.
Laflamme, C., Edwards, A.M., Bandrowski, A.E. and McPherson, P.S. (2021) Opinion: Independent third-party entities as a model for validation of commercial antibodies. New Biotech. (published on-line).
Bayati, A., Kumar, R., Francis, V., and McPherson, P.S. (2021) SARS-CoV-2 infects cells following viral entry via clathrin-mediated endocytosis. J. Biol. Chem. 296:100306. doi: 10.1016/j.jbc.2021.100306 Selected as a Journal of Biological Chemistry Editors’ Pick, 50-100 of the ~6600 papers the Journal publishes in a year are selected
Alshafie, W., Francis, V., Bednarz, K., Pan, Y.E., Stroh, T., and McPherson, P.S. (2020) Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion. J. Cell Biol. 219:e201904054 (Featured in the Faculty of 1000)
Kulasekaran, G., Chaineau, M., Piscopo, V.E., Verginelli, F., Fotouhi, M., Girard, M., Tang, Y., Dali, R. Lo, R. Stifani, S., and McPherson, P.S. (2021) An Arf/Rab cascade controls the growth and invasiveness of glioblastoma. J. Cell Biol. (In press).
Laflamme, C., McKeever, P.M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen, C. X.-Q., You, Z., Benaliouad, F., Gileadi, O., McBride, H.M., Durcan, T.H., Edwards, A.M., Healy, L.M., Robertson, J., and McPherson, P.S. (2019) Implementation of an antibody validation procedure and application to the major ALS/FTD disease gene C9ORF72 . eLife doi:10.7554/eLife.48363. (this manuscript led to two commentaries on antibody validation: Marx, V. (2020) Bye-Bye bunny: change makers bring on recombinant antibodies. Nature Methods 17:763-766; Baker, M. (2020) When antibodies mislead: the quest for validation. Nature 585:313-314)
Ioannou, M.S., Kulasekaran, G., Fotouhi, M., Morein, J.J., Han, C., Tse, S., Nossova, N., Han, T., Mannard, E. and McPherson, P.S. (2017) Intersectin-s interaction with the Rab13 exchange factor DENND2B facilitates recycling of epidermal growth factor receptor. EMBO Rep. 18:2119-2130 (a graphical summary of this manuscript was used for the cover of the corresponding issue of the journal, my 8th such cover).
Han C, Alkhater R, Froukh T, Minassian AG, Galati M, Liu RH, Fotouhi M, Sommerfeld J, Alfrook AJ, Marshall C, Walker S, Bauer P, Scherer SW, Riess O, Buchert R, Minassian BA, McPherson PS. (2016) Epileptic Encephalopathy Caused by Mutations in the Guanine Nucleotide Exchange Factor DENND5A. Am J Hum Genet. 99(6):1359-1367. doi: 10.1016.
Ioannou, M.S., Bell, E.S., Girard, M., Chaineau, M., Hamlin, J.N.R., Daubaras, M., Monast, A., Park, M., Hodgson, L., and McPherson, P.S. (2015) DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior. J. Cell Biol. 208: 629-648. (Featured in a JCB Podcast called biobytes, in which the journal highlights one significant paper appearing in each issue. A movie from this manuscript, imaging Rab13 activation at the leading edge of a migrating cell, was posted by the Journal on their Facebook page and received > 34,000 views. Featured in the Faculty of 1000).
Schreij, A.M.A., Chaineau, M., Ruan, W., Lin, S., Barker, P.A., Fon, E.A., and McPherson, P.S. (2015) LRRK2 functions in concert with clathrin-light chains to limit Rac1 activation and control actin cytoskeleton dynamics. EMBO Reports. 16: 79-86.
Ritter, B, Murphy, S., Dokainish, H., Girard, M., Gudheti, M.V., Koslov, G., Halin, M., Philie, J., Jorgensen, E.M., Gehring, K., and McPherson, P.S. (2013) NECAP 1 regulates AP-2 interactions to control vesicle size, number and cargo during clathrin-mediated endocytosis. PLoS Biology. 11: e1001670.
Girard, M., Larivière, R., Parfitt, D.A., Deane, E.C., Gaudet, R., Nossova, N., Blondeau, F., Prenosil, G., Vermeulen, E.G.M., Duchen, M.R., Richter, A., Shoubridge, E.A., Gehring, K., McKinney, R.A., Brais, B., Chapple, J.P. and McPherson, P.S. (2012) Mitochondrial dysfunction and Purkinje cell loss in Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay (ARSACS). Proc. Natl. Acad. Sci., USA 109: 1661-1666. (Selected as Novel and Newsworthy when presented at the American Society for Cell Biology, December 2011, coverage in multiple newspapers including a full-page feature in the Globe & Mail, reported by multiple radio and TV stations, subject of a Nature News and Views, Narenda, D.P. and Youle, R.J. (2012) Neurodegeneration: Trouble in the cell's powerhouse, Nature, 483: 418-419).
Allaire, P.D., Marat, A.L., Dall'Armi, C., Di Paolo, G., McPherson, P.S.*, and Ritter, B*. (2010) The connecdenn DENN domain: A GEF for Rab35 mediating cargo-specific exit from early endosomes. Mol. Cell. 37: 370-382. *=co-corresponding author.
Poupon, V., Girard, M. Legendre-Guillemin, V., Thomas, S., Bourbionniere, L., Philie, J., Bright, N.A. and McPherson, P.S. (2008) Clathrin-light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc. Natl. Acad. Sci., USA, 105:168-173. (selected by the Faculty of 1000)
Allaire, P.D., Ritter, B., Thomas, S., Burman, J.L., Denisov, A.Yu., Legendre-Guillemin, V., Harper, S.Q., Davidson, B.L., Gehring, K. and McPherson, P.S. (2006) Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis J. Neurosci. 26:13,202-13,212.
Blondeau, F., Ritter, B., Allaire, P.D., Wasiak, S., Girard, M., Hussain, N.K., Angers, A., Legendre-Guillemin, V., Roy, L., Boismenu, D., Kearney, R.E., Bell, A.W., Bergeron, J.J.M., and McPherson, P.S. (2004) Tandem mass spectrometry analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling. Proc. Natl. Acad. Sci., USA 101:3833-3838. (selected by the Faculty of 1000)
Ritter, B., Philie, J., Girard, M., Tung, E.C., Blondeau, F., and McPherson, P.S. (2003) Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif. EMBO Reports 4:1089-1093.
Wasiak, S., Legendre-Guillemin, V., Puertollano, R., Blondeau, F., Girard, M., de Heuvel, E., Hussain, N.K., Boismenu, D., Bell, A.W., Bonifacino, J.S. and McPherson, P.S. (2002) Enthoprotin, a novel clathrin-associated protein identified through subcellular proteomics. J. Cell Biol.158:855-862.
Metzler, M., Legendre-Guillemin, V., Gan, L., Chopra, V., Kwok, A., McPherson, P.S., and Hayden, M.R. (2001) HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and AP2. J. Biol. Chem. 276:39271-39276.
Hussain, N.K., Jenna, S., Glogauer, M., Quinn, C.C., Wasiak, S., Kay, B.K., Stossel, T.P., Lamarche-Vane, N., and McPherson, P.S. (2001) The endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP. Nat. Cell Biol. 3:927-932.
Simpson, F.S., Hussain, N.K., Qualmann, B., Kay, B.K., Kelly, R.B., McPherson, P.S., and Schmid, S.L. (1999) SH3 domain-containing proteins function at distinct steps in clathrin-coated vesicle formation. Nature Cell Biol. 1:119-124.
Hussain, N.K., Yamabhai, M., Ramjaun, A.R., Guy, A.M., Baranes, D., O'Bryan, J.P., Der, C.J., Kay, B.K., and McPherson, P.S. (1999) Splice variants of intersectin are components of the endocytic machinery in neurons and non-neuronal cells. J. Biol. Chem. 274:15671-15677.
de Heuvel, E., Bell, A.W., Ramjaun, A.R., Wong, K., Sossin, W.S., and McPherson, P.S. (1997) Identification of the major synaptojanin-binding proteins in brain. J. Biol. Chem. 272:8710-8716.