Confocal Imaging Facility
Located in room D28 of the Lyman Duff Medical Building, the Department of Microbiology and Immunology is equipped with a Fluoview 1000 laser-scanning confocal microscope and associated computing platform from Olympus. The FV1000 microscope in our department contains 458/488/515/543/633 nm wavelength lasers allowing for simultaneous multi-colour imaging, 10-100x objectives, live imaging capability and has been recently upgraded (2014-2015) with high resolution gadolinium detectors, a mechanical stage and a new computer equipped with stitching software for whole organ image capture. In addition, the microscope has been recently been equipped with an LED light source for epifluorescent microscopy to meet a range of experimental needs.
Laser-scanning confocal microscopy is a technology that allows for high resolution, optical sectioning of individual cells in culture or embedded within tissues. The wide range of fluorescent probes and detectors enables rapid analysis of multiple parameters within one sample. The advantage of this technology over wide-field epifluorescence microscopy is that it uses point illumination to enhance image resolution and control the depth of the focal plane within tissues. The use of lasers of different wavelengths to scan tissues and excite fluorescent cell labels both horizontally and vertically allows 3D rendering of cells within whole tissues. Using this instrument, investigators can accurately measure cell size and study cell morphology, membrane and intracellular protein expression as well as cell division and death. A particular benefit of this technology is that it combines these cellular parameters with detailed information about the location of specific cell types within complex tissues. This information elucidates how intercellular interactions impact organ-specific states of health and disease. Check the tutorial on the basics of confocal immunofluorescence microscopy.
Appointments to use this instrument are taken using an on-line booking system. Services provided by this facility including training of new users to ensure sufficient background knowledge to successfully design experiments, operate the equipment properly and with confidence. The facility also offers technical consultation on experiment design and analysis.
Training and Fees
Individuals interested in using our facility must receive on-site training with our imaging consultants MIA CELLAVIE Inc. prior to gaining room access and microscope use. Training sessions will take 6-8 hours and are $560/person (one additional person may be trained per session for an additional $130). This one time non-refundable fee, paid up front, will be recouped by the user in the form of 25 non-consecutive hours of free use.
The cost of additional use is $20/hour for Full and Associate Members of the Department and $40/hour for Non-members. For more training details or to schedule an appointment, please contact our Facility Manager:
Dr. Ghislaine Fontes
Duff Medical Building, Room 402
ghislaine.fontes [at] mcgill.ca (Email)
Dr. Ghislaine Fontes, Facility Manager
Duff Medical Building, Room 402
ghislaine.fontes [at] mcgill.ca (Email )
Dr. Irah King, Director
Duff Medical Building, Room 402A
irah.king [at] mcgill.ca (Email )