Protocols & Image Analysis

PROTOCOLS
 

Kohler Alignment [PDF]

Lens Cleaning Protocol [PDF]

Coverslip Acid Wash [PDF]

Bead Sample Preparation for PSF Measurements [PDF]

Fibronectin Coating and Plating Cells [PDF]

Fluorescence Labelling Protocol [PDF]

Immunofluorescence Staining on Paraffin Slides [PDF]

GFP-Paxiliin Cell Growth Media [PDF]

Quantitative Imaging Checklist [PDF]

How to build a microscope incubator with cardboard and bubble wrap [Website]

Imaging/Microscopy Protocols (Cold Spring Harbor) [Website]

Zeiss Literature & Guided Tour [zip]

 

IMAGE ANALYSIS
 

AquityXpress - Molecular Devices

Cell Profiler - FREEWARE - Image Analysis Software

Go Figure - FREEWARE - visualizing and tracking in multidimensions

ImageJ - FREEWARE

McMaster Biophotonics ImageJ suite - FREEWARE MIPAV (Medical Image Processing, Analysis, and Visualization)

MetaMorph Software Information -Molecular Devices

MetaXpress - Molecular Devices

MetaXpress Upgrades - Molecular Devices

NLM Insight Segmentation and Registration Toolkit - FREEWARE - segmentation and registration in multidimensional image series.

 

QUALITY CONTROL
 

Measuring and Interpreting PSF

Live-Cell Microscopy: Tips and Tools

Epifluorescence Microscopy

International test results for objective lens quality, resolution, spectral accuracy, and spectral separation for confocal laser scanning microscopes

Calibration of wide-field deconvolution microscopy for quantitative fluorescence imaging

Quantitative Confocal Microscopy: Beyond a Pretty Picture

Mercury free microscopy: an opportunity for core facility directors

A Quantitative Measure of Field Illumination

Any Way You Slice It - A Comparison of Confocal Microscopy Techniques