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LaVision Light Sheet UltraMicroscope

Selective plane illumination microscope for 3-dimensional imaging. Equipped with a 2nd light sheet that excites the identical specimen area from the opposed direction to improve the fluorescence homogeneity.

The Ultramicroscope II allows in vivo imaging as well as the imaging of cleared samples. All optical and mechanical components are compatible to most clearing solutions [DBE, Clarity, SeeDB] and water.

(Located in BT-105)

Triple Sheet Optics

For generating three laser sheets perpendicular to the direction
of detection. Width, Rayleigh length, focus and thickness of
the three light sheets can be set by software.
Min. thickness: <4.5 μm @ 488 nm for one individual sheet
Focus range: > ±5 mm from center position of the sample for
all usable imaging solutions

Motorized Tube Lens

For correcting the spectral focus shift of the detection optics
Spectral width: 450-700 nm for all usable imaging solutions

Continuous Shift of the Sheet Focus Position

For shifting the focus of the three light sheets through
the sample during the image acquisition.

Motorized Laser Attenuator

Attenuation by a continuously variable metallic ND filter

Imaging Chamber

Cuvette made of Quartz glass including sample holder.
Motorized XYZ-stage for moving imaging chamber and
sample holder.
Travel range: 10 x 10 x 10 mm³

Andor Neo sCMOS camera

Scientific low noise CMOS camera
Sensor: sCMOS
Pixel: 2560 x 2160
Pixel size: 6.5 x 6.5 μm²
Sensor size: 16.6 x 14 mm²
Full well capacity: 25000 e-
Read out noise: typ 1 e- rms @ 200 fps
Quantum efficiency: 57% @ 575 nm
Spectral range: 370…1100 nm

ImSpector Software

Acquisition of multi-dimensional data stacks.
Parameters (x, y, z, t, λexcitation, λemission) can be arbitrarily
combined.
Various mathematical evaluation algorithms and
multi-dimensional linear spectral-unmixing routines.

Olympus MVX-10 Zoom Body for Ultramicroscope

The variable 10x zoom body provides magnification steps
from 0.63x to 6.3x.

Olympus MVPLAPO 2x Dry lens including 2 LaVision BioTec dipping caps
Magnification: 2.0x
Numerical aperture: 0.50
Working distance:
6 mm with medium length dipping cap
4 mm with long dipping cap

Laser Module Gen. II

488-85 Laser for LVBT Laser Module Gen. II
Wavelength: 488 nm
Power: ≥ 85 mW
Beam quality [M²]: < 1.2
Spectral width: < 2 nm
Laser class: 3B

561-100 Laser for LVBT Laser Module Gen. II
Wavelength: 561 nm
Power: ≥ 100 mW
Beam quality [M²]: < 1.2
Spectral width: < 2 nm
Laser class: 3B

639-70 Laser for LVBT Laser Module Gen. II
Wavelength: 639 nm
Power: ≥ 70 mW
Beam quality [M²]: < 1.2
Spectral width: < 2 nm
Laser class: 3B

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