On an Olympus IX 83 inverted microscope
(BT-314)
Light Sources |
Objectives |
Fluorescence
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Features |
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Widefield LED Illumination, 405nm/470nm/590nm/635nm |
Olympus Plan-Apo 100x/1.40NA Oil |
Quad Band filter cube(DAPI/FITC, STAR488/Cy3/Cy5, STAR635P) |
A 4-channel confocal- and STED-microscope for live-cell imaging, including RESCue for minimizing bleaching and light dose |
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Excitation lasers: 405, 488, 561 and 640nm |
Olympus Plan-Apo 60x/1.2NA water |
FLIM hardware and software integration |
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Two pulsed STED lasers @ 595nm, 775nm |
Stage top incubator chamber with temperature, CO2 and humidity control (OKO lab) |
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All lasers are equipped with an Acousto-Optic Modulator (AOM) for ultrafast switching. |
Detectors: four Avalanche Photo Diodes (APDs) with ultra-high detection efficiency |
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