This system enables high-speed confocal imaging and total internal reflection fluorescence (TIRF) microscopy.
Typical applications include:
- Live-cell imaging and dynamic cellular processes
- Membrane and surface protein imaging using TIRF
- High-speed fluorescence imaging
- Multi-channel cellular imaging
EQUIPMENT FEATURES | SPECIFICATIONS |
|---|---|
| Stand model | Observer Z1 |
| Features and Applications | Brightfield, DIC 4D Imaging (XY, Z-stack, Time-lapse) Piezo Stage Multi-Channel (up to 3 dyes simultaneous imaging) FRET FRAP Live cell imaging. Tiling. Multipoint Definite Focus Piezo stage TIRF Inserts: Slides, culture cell plates and dishes |
| Light Sources | Laser lines: 405nm (50mW), 488nm (100mW), 561nm (75mW) HXP 120V |
| Objectives | 10x Plan Apo, DIC, NA 0.45 20x Plan Apo, DIC, NA 0.8 40x Plan Apo, DIC, NA 1.4 Oil 63x/ Alpha Plan Apo, NA 1.46, Oil, Corr, TIRF |
| Fluorescence Cubes | FS 38 BP 450-490 / BP 500-550 (eGFP) FS 43 BP 532-558 / BP 570/640 (Cy3) FS 49 G 365 / BP 420-470 (DAPI) FS 77HE TBP 483+564+642 / TBP 526+601+688 (GFP-mRFP+Alexa 633) LSM Duo T20/R80 UV Chroma TRF89901V2 TBP 405+488+561+640 nm / TBP 450+525+590+700 (DAPI, eGFP, Cy3, Alexa 633) |
| SD Emission filters | BP 450/50 BP 525/50 DBP 527/54+645/60 BP 629/62 BP690/50 Quad Emitter 446/523/600/677 |
| Software | Zen |
| Detectors | Photometrics Prime 95B Axiocam 506Mono |
| Location | MS2-094c |
