Mechanisms driving the increase in cell growth in developing leukemia are not fully understood. We focused on epigenomic regulation of this process by analyzing the changes of chromatin marks and gene expression in leukemic cell clones as they progressed toward increased proliferation in a mouse model of acute myeloid leukemia (AML). This progression was characterized by gradual modulation of chromatin states and gene expression across the genome, with a surprising preferential trend of...

Antimicrobial susceptibility testing (AST) technologies that rapidly identify pathogenic bacteria and their resistance phenotypes are critical in addressing the antimicrobial resistance crisis, enabling timely and precise antibiotic treatment decisions. We present a modular automated platform based on nanoplasmonic colorimetry in microfluidics for parallel bacterial identification and phenotypic profiling of AST (QolorPhAST), achieving an eightfold enhancement in detection rapidity. QolorPhAST...

In vitro enzyme assays provide sensitive and quantitative approaches to studying activity, identifying substrates or performing inhibitor analysis. These approaches can help to study enzymes that regulate different cellular processes in an organism. In addition, enzyme assays can be used to screen protein inhibitors to identify novel drug candidates. In eukaryotes, phosphatidylinositol kinase and phosphatase are enzymes that regulate the level of phosphorylated phosphatidylinositol, which...

BACKGROUND: Tuberculosis elimination requires expanded use of preventive treatment. However, adherence to the tuberculosis infection cascade of care is often an issue, notably in vulnerable groups such as migrants. We aimed to assess a person-centred approach to tuberculosis screening and treatment using facilitators, comparing strategies for tuberculosis prevention among new migrants to Canada.

CONCLUSIONS AND RELEVANCE: In this cross-sectional study of travelers' diarrhea antimicrobial resistance patterns, there was marked variability of nonsusceptibility to 2 major classes of antibiotics commonly used for treating travelers' diarrhea among global regions. Antimicrobial susceptibility from culture should be obtained when possible, including after pathogen detection by culture-independent methods. These findings may help inform strategies for self-treatment and clinician management of...

CONCLUSION: Thus, CBHM-Ferlo strategy improved the care of childhood illness in Matam in 2024, and above all highlights the added value of One Health and transdisciplinary approaches.

Due to climate change and human interventions, there has been an increase in D. immitis infections, underscoring the necessity for monitoring the spread and extent of resistance. In our prior research, we introduced a rapid test utilizing four predominant SNP markers at loci 15709 (SNP1), 30575 (SNP2), 21554 (SNP3), and 9400 (SNP7) linked to ML resistance. Our findings highlighted SNP1 and SNP2 as potent predictive markers, offering suitability for the rapid detection and monitoring of drug...

CONCLUSIONS: An evaluation of pharmacovigilance data from 2 different sources revealed no evidence of greater proportional reporting for neurological AEs specific to the use of eprinomectin-containing products in cats than for other MLs or emodepside.

BACKGROUND: As the result of systemic and structural barriers, hepatitis C virus (HCV) continues to disproportionately affect people who inject drugs, those in prison, Indigenous peoples, immigrants from HCV-endemic countries, and gay, bisexual and other men who have sex with men in Canada. Point-of-care antibody and RNA testing improve access to HCV testing and enable single-visit diagnosis and treatment initiation, yet robust, context-specific strategies are needed to scale these technologies...

Trypanosoma cruzi, the causative agent of Chagas disease, poses a significant health challenge due to limited therapeutic options and an incomplete understanding of its biology. Approximately half of the genome encodes hypothetical proteins with unknown functions, underscoring the need for systematic functional annotation. Protein-protein interactions (PPIs) underpin essential cellular processes, yet no large-scale PPI map has been developed for T. cruzi ─a critical gap that impedes both...

CONCLUSIONS: These findings suggest that sex may not be a major factor in vaccine response in pediatric populations, but may be more likely to be detected in influenza vaccine trials with a non-influenza vaccine control group.

Loiasis remains one of the least prioritised filarial infections, yet its neglect undermines onchocerciasis elimination and limits progress toward global NTD goals. Recognising Loa loa as a neglected tropical disease would unlock resources, promote safe mass drug administration, and accelerate the World Health Organization's 2030 targets.

INTRODUCTION: For more than 15 years, artemisinin-based combination therapy (ACT) and atovaquone-proguanil have been recommended as treatment of Plasmodium falciparum malaria in most nonendemic countries. However, new challenges have emerged that complicate the management of travelers with malaria, including the occurrence of artemisinin-related hemolysis and the increasing numbers of treatment failure possibly related to drug resistance.

Salmonella is a significant global enteric pathogen with a high incidence of multidrug resistance (MDR), which limits therapeutic options and necessitates the development of novel treatments. Antisense peptide nucleic acids (PNAs), which target essential bacterial genes, have demonstrated potential as antibacterial agents by effectively inhibiting the growth of various pathogens. Peptidoglycan (PG) has historically been one of the central targets for antibiotics in the ongoing battle against...

Atopic dermatitis (AD) in infants is associated with increased risk of developing other allergic diseases, including asthma. This progression is known as atopic march (AM). We established a murine AM model by first inducing AD-like skin inflammation to house dust mite (HDM) allergen. Mice were subsequently challenged with HDM delivered to the lung. Our data show that epicutaneous sensitization with HDM increased serum IgE, ear thickness, and immune cell infiltration into the skin, accompanied by...

Rift Valley fever virus (RVFV) is a mosquito-borne phlebovirus that causes severe febrile and hemorrhagic illness in humans. In September 2025, an outbreak in northern Senegal led to 119 confirmed infections and 15 deaths as of October 7, 2025. We performed rapid genomic sequencing to characterize the virus responsible for this epidemic. RNA from RT-qPCR-confirmed samples was sequenced using the Twist Comprehensive Viral Research Panel on an Illumina iSeq 100 platform. Consensus genomes were...

Genome three-dimensional organization is essential for eukaryotic gene expression. The chromosomes of the pathogen Trypanosoma brucei contain hundreds of silent variant surface glycoprotein (VSG) genes in subtelomeric regions. T. brucei transcribes a single VSG gene and periodically changes the VSG expressed, altering its surface coat to escape host antibodies by antigenic variation. We show that T. brucei core and subtelomeric chromosome compartments are separated by distinct boundaries and...

Trypanosoma cruzi possesses hundreds of genes associated with pathogenesis. The extent and organization of this diverse gene repertoire, expression, and role in infection remain unclear. Using accurate long-read sequencing and chromatin conformation capture, we assembled T. cruzi Sylvio X10 strain chromosomes from telomere-to-telomere. The genome provides accurate organization of multigene family genes, confirming their distribution in expanded clusters or scattered throughout the chromosomes....

Host specificity is a critical feature of the survival and proliferation of parasites. In the context of interactions with the host, numerous mechanisms have been identified, particularly in parasitic helminths, that enable manipulation of the host immune system to enhance their own survival. The evolutionary history of these interactions often results in hosts becoming disease-tolerant or asymptomatic, even when burdened with a high number of worms, until a disruption in the host's immune...

Understanding and identifying protein-ligand interactions is essential for elucidating fundamental biological processes and developing translational applications such as vaccine and drug targets. Yeast surface display (YSD) systems expressing genome-wide or combinatorial libraries in Saccharomyces cerevisiae are powerful tools to identify protein-ligand interactions in an unbiased fashion. In the following protocol, we couple the YSD system expressing a genome-wide library of the protozoan...