Available Technology
Our lab is equipped with a variety of equipment and resources that enable us to develop novel cell lines and manufacturing processes for the production, purification and characterization of viral vectors and vaccines.
BioRad QX200 Droplet Digital PCR System
The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Sartorius Ambr® 250 Modular
Our control units enable us to operate bioreactors of different capacities, up to 250 mL. These are suitable to generate enough material for small scale applications and modify conditions for scaling up purposes.
GE ÄKTA - Avant Purification System
ÄKTA avant is a preparative protein purification chromatography system designed for fast and secure development of scalable methods and processes.
Waters - HPLC Alliance System
Reliable and flexible workhorse for HPLC separations. The advance software functionality simplifies method development and reporting.
BioRad - Chemidoc XRS+ System
Based on CCD technology and modular options, the imaging system accommodates a wide range of samples and supports multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence.
Cell-based Expression Platforms
Our strategic focus regarding expression platforms is mainly centered around eukaryotic cell-based systems, namely HEK293, VERO, and insect-derived cell lines with the option of using the baculoviral expression system. The relevance of these platforms resides in their widespread use within the industrial sector.
HEK293
HEK293 is a cell line originally derived from human embryonic kidney. Besides being commonly used in cancer research, it has become an iconic cell line for the production of adenoviral-based and retroviral-based viral vectors.
Insect cells - Baculovirus
Insect cells used for the expression of recombinant proteins are derived from insects susceptible to the infection of Baculoviruses. By replacing certain viral genes with genes coding for proteins of interest, it is possible to produce a great variety of recombinant products.
Vero
The Vero cell line is the most widely used continuous cell line for the production of viral vaccines, including those against influenza, Japanese encephalitis, polio, and rabies, amongst others. The original Vero cell line was derived in 1962 from the kidney of an African green monkey. The cells are interferon deficient, which could explain their permissiveness to viral infection.
