Advanced BioImaging Facility
Location:
McGill Life Sciences Complex
Unit:
Faculty of Medicine & Health Sciences
Areas of expertise:
Live cell imaging, FRAP, FLIM, FRET, FCS, image correlation spectroscopy, TIRF, spectral imaging, high content screening, multi-photon imaging, laser capture micro-dissection, cellular image analysis, custom image analysis.
Link(s):
Instrument(s):
Point scanning confocal
Spinning disc confocal
Light sheet
Super-resolution microscope
Projects:
- Confocal microscopy
- Multiple fluorophore imaging
- Co-localization
- 3D Imaging
- Deconvolution
- 4D dynamic live cell imaging
- Cell migration and tracking
- Laser micro-dissection (PALM)
Professional activities:
- Protein diffusion/interactions in living cells (FCS)
- Cell surface imaging with < 100 nM z-resolution (TIRF)
- Fluorescence Recovery after Photobleaching (FRAP)
- Fluorescence Resonance Energy Transfer (FRET)
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Spectral imaging to eliminate autofluorescence or image 6-8 dyes simultaneously
- Image Correlation Microscopy of protein dynamics and interactions (ICS, RICS, STICS)
Specialization:
- 3D reconstruction
- Filament tracing (i.e. neuron dendrites)
- Protein colocalization
- Cell tracking
- Expression (intensity) analysis
- And more!