Robotics SAGIAN System
Our SAGIAN automated system has recently been upgraded thanks to a large infrastructure grant from the Canadian Foundation of Innovation (CFI), awarded to the Integrated Quantitative Bioscience Initiative (IQBI). The robotic system now boasts a brand-new SCARA arm, the latest Biomek pinning-liquid handler, a new Thermocycler and a high-performance CCD camera in a custom-made black box. It can perform (almost) any experiment, including liquid handling, colony pinning, shaking, incubating, sealing, running PCR, imaging over long periods of time...
Module Description |
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Automated Workstation (Biomek NEW FXp, Beckman Coulter) |
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Incubator (Cytomat 6001, Kendro) - Accommodates up to 125 standard plates and 30 deep-well plates - Incubation from 10 to 50ºC. - CO2 supply possible for cell culture - Agar plates can be incubated (and manipulated) upside down to prevent condensation on lid. |
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Plate Reader (DTX880, Beckman Coulter) - For absorbance, luminescence, fluorescence intensity, fluorescence polarization and time-resolved fluorescence. - Heated chamber (from 4ºC above ambient up to 45ºC). - Reads from top or bottom of 6- to 384-well plates. - Current filter sets include 260, 340, 370, 405, 450, 485, 535, 595 and 620 nm for excitation and 535, 595 and 625 nm for emission. - Can perform endpoint, multiple wavelength, kinetic, or area scans. |
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Grashopper3 USB 3.0 CCD Camera (Point Grey) |
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Plate Washer (ELx405 select, Biotek Instruments) - Dispenses from top and washes from bottom. - Flow rate is adjustable for sensitive assays. |
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Carousel (Beckman Coulter) - Accommodates up to 90 standard plates and 40 deep-well plates or tip boxes. |
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Thermocycler (TRobot , Biometra) |
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Robotic Arm (SCARA, Beckman Coulter) |
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Dispenser (Multidrop384, Titertek) - 8-channel head to dispense 5 to 395 μl from a single or multiple reagent bottles |
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Lidding Station (Beckman Coulter) - Accommodates up to 6 lids. - Maintains the lid-plate association. |
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Plate Shaker (Beckman Coulter) - Configured with 6-plate locations. |
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Plate Sealer (ALPS 300, from Abgene) - Used to seal plates for storage or to prevent evaporation or contamination during incubation or shaking. |
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Tip Lift and Disposal Unit (Beckman Coulter) - Accommodates up to 96 200 μl tip boxes. |