PACB offers a wide range of services for respiratory disease investigators such as:
Cryovials of 1 million frozen, viable primary Human Bronchial Epithelial (HBE) cells
Highly-differentiated HBE cells on porous filters (transwells, snapwells)
Primary HBE cells cultured on plastic flasks (T25)
Specialized cell culture media: BEGM (Bronchial Epithelial cell Growth Medium) for culture on plastic and ALI (Air-liquid interface) for culture on porous filters
Transepithelial electrophysiological measurements (i.e. for drug discovery project)
Personalized technical support service
Prices and stock of cells are subjected to modifications. For more details, please contact us.
Detailed donor information are provided to users for each lot of primary HBE cells.
Quality control
PACB provides primary HBE cells that are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells are tested negative for mycoplasma, bacteria, yeast, and fungi, as well as for HIV, hepatitis A, B and hepatitis C.
Image by Julie Goepp.Image by Julie Goepp.
Sex
Smoker
For CF donors:
Age
BMI
Mutation
Ethnicity
Medication
Antibiogram
Diagnosis
Cause of death
CF associated diabetes
Other pathology
Serology
Pancreatic insufficiency
FEV1, FEV1% predicted
PACB performs the following verification:
Cell purity
Viability
Capacity of differentiation into pseudostratified mucociliary epithelium
Transport function (Ussing chamber)
Validation of CF genotype
Free of bacteria, fungi and mycoplasma
Serology: absence of viruses (HIV-1,2; Hepatitis A, B, C; Hantavirus; HTLV-1,2)
PACB also offers a variety of human primary airway cells and specimens, from normal and diseased donors (ie. CF, COPD, asthma, chronic bronchitis, emphysema):
Bronchial specimens available:
HBE (Human Bronchial Epithelial cells)
HTG (Human Tracheal Gland cells)
Airway fibroblasts
Airway smooth muscle cells
Sputum
Image by Julie Goepp.
Other specimens available:
Nasal brushes
Nasal polyps
Nasal internal turbinate
Image by Julie Goepp.
Tissue bank:
Image by Julie Goepp.
Airway Tissue:
Trachea
Superior bronchus
Inferior bronchus
Smooth muscle
Parenchyma (alveolar tissue)
Endothelium
Sections from fixed tissue in parrafin blocks or OCT available
Snapfrosts of fresh tissues available
Polarized cells (on porous filters):
HBE cells
Nasal cells
Cytospins:
Nasal brushes
HBE cells
Transepithelial electrophysiological measurements
On a fee-for-service basis, PACB performs for you transepithelial electrophysiological measurements of well-differentiated epithelial cells for basic research and drug discovery projects. Contact us for more details.
Electrophysiological measurements
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