TIRF-Spinning Disk Spectral Diskovery System

Total Internal Reflection Fluorescence (TIRF) microscope for looking within ~100 nm of the microscope slide. Specifically well suited for endocytosis, exocytosis, adhesion and membrane protein/lipid studies.

Features:

  • Total Internal Reflection Fluorescence (TIRF)
  • Variable pinhole Spinning Disk Confocal
  • Rapid simultaneous two colour imaging on EMCCD
  • Environmental Control for live cell imaging


Light Sources:

  • Halogen Lamp for Transmitted Light
  • Wide Field Microscopy - X-Cite 120 LED (370-700nm), 100W
  • Diode Laser - 488nm with FLCRtm control system
  • Diode Laser - 561nm
  • Diode Laser - 642 nm


Objectives:

  • 10X N-PLAN, NA=0.25
  • 20X HC PLAN APOCHROMAT, NA=0.70,
  • 63X HCX PLAN APOCHROMAT, NA=1.47, OIL, Corr, TIRF


Fluorescence Filter Cubes:

  • EGFP (ET 525/50nm)
  • mCherry (ET 620/60nm)
  • EGFP/mCherry Dual Cube

Fluorescence Probes:

  • EGFP
  • mCherry
  • Cy5/IR-FP

Detectors:

  • Hamamatsu ImageEMX2 EM-CCD Cameras

Additional Features:

  • DIC Optics
  • High resolution sequential multi-colour imaging


Location:

  • Located in Bellini Building, Room 147.