Abberior STED Super-Resolution Microscope

This Abberior STED system allows researchers to implement this relatively new super resolution technique in two different colours, while also allowing for standard confocal imaging. For inquiries about access to the STED, visit our Contact Us page.

STimulated Emission Depletion (STED) Microscopy   This imaging technique allows researchers to achieve resolution below the diffraction limit of light (about 200nm). This works by selectively deactivating fluorophores, resulting in only a very small area of illumination at the focal point.

Features:

• Resolution up to 50nm
• Two STED excitation lasers, one depletion laser, and four confocal excitation lasers
• Simultaneous 2-colour STED
• 3 APD detectors (up to 3 simultaneously detected wavelengths)
• RESOLFT for live-cell imaging
   

Available STED Excitation Lasers:                                Available STED Depletion Laser:

• 561 nm                                                                                             775 nm, 1.5 W
• 640 nm
   

Available Confocal Excitation Lasers:

• 405 nm
• 488 nm
• 561 nm
• 640 nm
   

Excitation Objectives:

• 100x PLAN APOCHROMAT, NA= 1.47, Oil
   

Fluorescence Components:

• Filter module GFP (dichroic 550 nm + filter 525/25)
• Filter module Cy3 (dichroic 640 nm + filter 605/25)
• Filter bloc Cy5 (filter 685/35)
• 2 other filter blocs available: YFP (filter 560/50) and mKate (filter 660/80)
   

Optional Incubation Systems:

• Not available