1- Protein Expression (as part of an antibody production project only):
His- or GST-tagged fusion in E. coli
Subcloning into expression vector tagging available
Antigen expression and purification
2- Antibody Production (in collaboration with local animal facilities):
Rabbit or rat polyclonal antibodies (standard 80-day immunization)
Sera tested by Western Blotting
Service can start from any step of the process scheme and must include antibody generation (we are NOT a protein production facility)
The service is open to anyone.
Users planning to use the PEAP service must cian [at] mcgill.ca (submit a short proposal) describing the general scope of the planned research. Target start and end dates are strongly encouraged.