Primary Airway Cell Biobank



PACB offers a wide range of services for respiratory disease investigators such as:

  • Cryovials of 1 million frozen, viable primary Human Bronchial Epithelial (HBE) cells

  • Highly-differentiated HBE cells on porous filters (transwells, snapwells)

  • Primary HBE cells cultured on plastic flasks (T25)

  • Specialized cell culture media: BEGM (Bronchial Epithelial cell Growth Medium) for culture on plastic and ALI (Air-liquid interface) for culture on porous filters

  • Transepithelial electrophysiological measurements (i.e. for drug discovery project)

  • Personalized technical support service


Prices and stock of cells are subjected to modifications. For more details, please contact us.


Detailed donor information are provided to users for each lot of primary HBE cells, such as: 



Quality control


PACB provides primary HBE cells that are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells are tested negative for mycoplasma, bacteria, yeast, and fungi, as well as for HIV, hepatitis A, B and hepatitis C.


Image by Julie Goepp.
Image by Julie Goepp.

Sex Smoker For CF donors:
Age BMI Mutation
Ethnicity Medication Antibiogram
Diagnosis Cause of death CF associated diabetes
Other pathology Serology Pancreatic insufficiency
    FEV1, FEV1% predicted


PACB performs the following verification:

  • Cell purity
  • Viability
  • Capacity of differentiation into pseudostratified mucociliary epithelium
  • Transport function (Ussing chamber)
  • Validation of CF genotype
  • Free of bacteria, fungi and mycoplasma
  • Serology: absence of viruses (HIV-1,2; Hepatitis A, B, C; Hantavirus; HTLV-1,2)



PACB also offers a variety of human primary airway cells and specimens, from normal and diseased donors (ie. CF, COPD, asthma, chronic bronchitis, emphysema):


Bronchial specimens available:


  • HBE (Human Bronchial Epithelial cells)
  • HTG (Human Tracheal Gland cells)
  • Airway fibroblasts
  • Airway smooth muscle cells
  • Sputum


Image by Julie Goepp.

Other specimens available:


  • Nasal brushes
  • Nasal polyps
  • Nasal internal turbinate


Image by Julie Goepp.

Tissue bank:


Image by Julie Goepp.

Airway Tissue:

  • Trachea
  • Superior bronchus
  • Inferior bronchus
  • Smooth muscle
  • Parenchyma (alveolar tissue)
  • Endothelium
    • Sections from fixed tissue in parrafin blocks or OCT available
    • Snapfrosts of fresh tissues available

Polarized cells (on porous filters):

  • HBE cells
  • Nasal cells


  • Nasal brushes
  • HBE cells





















Transepithelial electrophysiological measurements


On a fee-for-service basis, PACB performs for you transepithelial electrophysiological measurements of well-differentiated epithelial cells for basic research and drug discovery projects. Contact us for more details.

Electrophysiological measurements

Supported by:


McGill University is situated on the traditional territory of the Kanien’kehà:ka, a place which has long served as a site of meeting and exchange amongst nations. We recognize and respect the Kanien’kehà:ka as the traditional custodians of these lands and waters.



Back to top