PACB offers a wide range of services for respiratory disease investigators such as:
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Cryovials of 1 million frozen, viable primary Human Bronchial Epithelial (HBE) cells
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Highly-differentiated HBE cells on porous filters (transwells, snapwells)
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Primary HBE cells cultured on plastic flasks (T25)
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Specialized cell culture media: BEGM (Bronchial Epithelial cell Growth Medium) for culture on plastic and ALI (Air-liquid interface) for culture on porous filters
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Transepithelial electrophysiological measurements (i.e. for drug discovery project)
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Personalized technical support service
Prices and stock of cells are subjected to modifications. For more details, please contact us.
Detailed donor information are provided to users for each lot of primary HBE cells, such as:
Quality control
PACB provides primary HBE cells that are suitable for air-liquid interface studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels. Cells are tested negative for mycoplasma, bacteria, yeast, and fungi, as well as for HIV, hepatitis A, B and hepatitis C.
| Sex | Smoker | For CF donors: |
| Age | BMI | Mutation |
| Ethnicity | Medication | Antibiogram |
| Diagnosis | Cause of death | CF associated diabetes |
| Other pathology | Serology | Pancreatic insufficiency |
| FEV1, FEV1% predicted |
PACB performs the following verification:
- Cell purity
- Viability
- Capacity of differentiation into pseudostratified mucociliary epithelium
- Transport function (Ussing chamber)
- Validation of CF genotype
- Free of bacteria, fungi and mycoplasma
- Serology: absence of viruses (HIV-1,2; Hepatitis A, B, C; Hantavirus; HTLV-1,2)
PACB also offers a variety of human primary airway cells and specimens, from normal and diseased donors (ie. CF, COPD, asthma, chronic bronchitis, emphysema):
Bronchial specimens available:
- HBE (Human Bronchial Epithelial cells)
- HTG (Human Tracheal Gland cells)
- Airway fibroblasts
- Airway smooth muscle cells
- Sputum
Other specimens available:
- Nasal brushes
- Nasal polyps
- Nasal internal turbinate
Tissue bank:
Airway Tissue:
- Trachea
- Superior bronchus
- Inferior bronchus
- Smooth muscle
- Parenchyma (alveolar tissue)
- Endothelium
- Sections from fixed tissue in parrafin blocks or OCT available
- Snapfrosts of fresh tissues available
Polarized cells (on porous filters):
- HBE cells
- Nasal cells
Cytospins:
- Nasal brushes
- HBE cells
Transepithelial electrophysiological measurements
On a fee-for-service basis, PACB performs for you transepithelial electrophysiological measurements of well-differentiated epithelial cells for basic research and drug discovery projects. Contact us for more details.
Electrophysiological measurements
