|Manager:||Mark Hancock, Ph.D.|
|Web:||mark.hancock [at] mcgill.ca|
McGill SPR Facility
For applications including proteomics, drug discovery, immunogenicity, and food analysis, state-of-the-art Surface Plasmon Resonance (SPR) technology allows for label-free, real-time biomolecular interaction analysis. Using purified preparations or complex mixtures, binding interactions between proteins, lipids, carbohydrates, nucleic acids, small molecules, and cells/viruses can be examined quantitatively in their native state with low sample consumption. In a typical experiment, the ligand (‘bait’) is immobilized to a gold-plated sensor chip and repeated injections of analyte (binding partner ‘target’) are then flowed overtop. A variety of sensor chip surfaces (eg. dextran, streptavidin, antibodies) and immobilization chemistries (eg. amine, capture, thiol) are available to tailor each project. Analyte-ligand association and dissociation events are monitored by changes in molecular mass accumulation at the solid-liquid interface on the sensor chip surface. The acquired data can answer questions regarding binding specificity, stoichiometry, concentration, kinetics (ka, kd), thermodynamics, and overall affinity (KD=kd/ka). To discover novel interaction partners, unknown binding candidates can also be recovered from cellular/tissue lysates for subsequent identification by Mass Spectrometry (SPR-MS ligand fishing). As described at www.biacore.com, our facility is equipped with two BIACORE 3000 systems.
2012 / Research Institute of the MUHC: Deme, J.C., Miousse, I.R., Plesa, M., Kim, J.C., Hancock, M.A., Mah, W., Rosenblatt, D.S., & Coulton, J.W. Structural features of recombinant MMADHC isoforms and their interactions with MMACHC, proteins of mammalian vitamin B12 metabolism. Molecular Genetics & Metabolism 107:352-62. Article
2011 / Universite de Montreal: Graveline, R., Mourez, M.,Hancock, M.A.,Martin, C., Boisclair, S., & Harel, J.Lrp-DNA complex stability determines the level of ON cells in type P fimbriae phase variation. Molecular Microbiology 81:1286-1299.Article
2011 / Concordia University: Kornblatt, J., Kornblatt, J., & Hancock, M.A. The interaction of canine plasminogen with Streptococcus pyogenes enolase: they bind to one another but what is the nature of the structures involved?PLoS ONE 6(12):e28481. Article
2011 / McGill University: Mireuta, M., Hancock, M.A., & Pollak, M. Binding between insulin-like growth factor 1 and insulin-like growth factor-binding protein 3 is not influenced by glucose or 2-deoxy-D-glucose. The Journal of Biological Chemistry 286:16567-16573.Article
2010 / Montreal Neurological Institute & Harvard Medical School: O'Connor, K.C., C. Lopez-Amaya, D. Gagne, L. Lovato, N. H. Moore-Odom, J. Kennedy, L. Krupp, S. Tenembaum, J. Ness, A. Belman, A. Boyko, O. Bykova, J. K. Mah, C. A. Stoian, E. Waubant, M. Kremenchutzky, M. Ruggieri, M. R. Bardini, M. Rensel, J. Hahn, B. Weinstock-Guttman, E. A. Yeh, K. Farrell, M. S. Freedman, M. Iivanainen, V. Bhan, M. Dilenge, Hancock, M.A., D. Gano, R. Fattahie, L. Kopel, A. E. Fournier, M. Moscarello, B. Banwell, & Bar-Or, A. Anti-myelin antibodies modulate clinical expression of childhood multiple sclerosis. Journal of Neuroimmunology 223:92-99. Article
For user fees and to request an estimate, visit us on Science Exchange:
- ‘User’ fee includes instrument and labour: preventative maintenance, SPR materials, method development, routine analyses, data evaluation, project meetings, scientific reports, and manuscript preparations. ‘Sensor Chip’ fee is extra based upon GE Healthcare catalog prices - please inquire.
- Billed by the quarter, services are payable in half-day or full-day units regardless of experimental outcomes. A normal business day (9am - 5pm, Monday - Friday) constitutes 1 full unit of time.
- Prices are in Canadian dollars and subject to change without notice. Applicable taxes are not included.