Human antigen R in lung pathology - PHAR 396 Undergraduate Research Project Application Form

Supervisor's Name: Carolyn Baglole

Supervisor's Email: carolyn.baglole [at] mcgill.ca

Supervisor's Phone: 514-934-1934

Supervisor's Website:

Supervisor's department: Pharmacology

Supervisor's department (if none of the above):

Course number: PHAR 396 (Pharmacology)

Term: Winter 2018

Project start date: Monday, January 8, 2018

Project end date: Monday April 16, 2018

Project title: Human antigen R in lung pathology

Project description (50-100 words suggested): Cigarette smoke (CS) is the primary cause of chronic obstructive pulmonary disease (COPD), an incurable and prevalent respiratory disorder characterized by chronic inflammation. This inflammation is typified by increase in mediators such as interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2). CS may contribute to lung inflammation via human antigen R (HuR), a ubiquitously-expressed RNA-binding protein (RBP) that regulates the stability of mRNA that encodes for inflammatory proteins. CS also causes apoptosis and autophagy, 2 cell death mechanisms also implication in COPD pathogenesis. Whether HuR also regulates these cell death mechanisms in the lung is not known. We hypothesize that HuR promotes the expression of proteins involved in apoptosis and autophagy. Using in vitro models of smoke exposure, together with techniques such as qRT-PCR and western blot, this project will evaluate the contribution of HuR to CS-induced cell death mechanisms.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade will be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages).

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances.