Transcriptional regulation of genes associated with childhood asthma - HGEN 396 Undergraduate Research Project Application Form

Supervisor's Name: Anna Naumova

Supervisor's Email: anna.naoumova [at] mcgill.ca

Supervisor's Phone: (514) 934-1934 x35906

Supervisor's Website:

Supervisor's department: Human Genetics

Course number: HGEN 396 (Human Genetics)

Term: Winter 2018

Project start date: Monday, January 15, 2018

Project end date: Friday, April 27, 2018

Project title: Transcriptional regulation of genes associated with childhood asthma

Project description (50-100 words suggested): The human chromosomal region 17q12-q21 is associated with childhood asthma.  It harbors 5 protein-coding genes.  The goal of this project is to characterize the tissue-specific RNA isoforms (transcripts) of one of the candidate genes for asthma predisposition, the zona pellucida binding protein 2 (ZPBP2).  Hypothesis: the causal gene is differentially regulated in different tissues. Approach: RNA and DNA extraction and purification, analysis of RNA isoforms in different mouse organs and human cell lines using RT-PCR and sequencing.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor. Details of the evaluation scheme will be provided by HGEN to supervisors and applicants.

Project status: This project is open to applicant.

How students can apply / Next steps: For HGEN: After all of the parts of this application forms are completed and the hard copy is signed by the professor and the student, return the application form with a copy of your unofficial transcript to Mr Ross MacKay in the Department of Human Genetics (electronically or hardcopy; see www.mcgill.ca/humangenetics/about-us for contact information).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects; Biohazardous substances; Handling chemicals.