Generation of recombinant hTLR10-Fc - MIMM 397 Undergraduate Research Project Application Form

Supervisor's Name: Joaquin Madrenas

Supervisor's Email: joaquin.madrenas [at]

Supervisor's Phone: 3914

Supervisor's Website:

Supervisor's department: Microbiology and Immunology

Course number: MIMM 397 (Immunology)

Term: Fall 2014-2015

Project start date: September 2, 2014

Project end date: December 4, 2014

Project title: Generation of recombinant hTLR10-Fc

Project description (50-100 words suggested): Little is known about TLR10 due to the lack of animal models available to investigate its function.  It has been shown that TLR10 is highly homologous to TLR1 and TLR6; therefore it could potentially form heterodimers with TLR2.  The aim of this project is to construct a TLR10-Fc fusion protein, which can serve as a tool to generate fractions of the Staphlococcus aureus cell wall, to help identify potential TLR10 ligands and to explore TLR10 signaling pathways.  It can also be used as a potential therapeutic tool to alter TLR-mediated cytokine responses to S. aureus.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).

Project status: This project is taken.

How students can apply / Next steps: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Human subjects; Biohazardous substances.