Cytokine Production from Human Plasma Cells - MIMM 397 Undergraduate Research Project Application Form

Supervisor's Name: Dr. Amit Bar-Or

Supervisor's Email: amit.bar-or [at] mcgill.ca

Supervisor's Phone:

Supervisor's Website:

Supervisor's department: Microbiology and Immunology

Course number: MIMM 397 (Immunology

Term: Fall 2014-2015

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: Cytokine Production from Human Plasma Cells

Project description (50-100 words suggested): Increasing evidence in mice suggests that besides antibodies, plasma cell can regulate local immune responses through the production of cytokines. The aim of this project is to validate these observations in humans and test the cytokine profile of in-vitro induced human plasma cells. Methods: human plasma cells will be induced by either a T-cell dependent (CD40L+IL-21+Anti-BCR) or T-cell independent (CpG+IL-21) approach. ELISA and intracellular cytokine staining will be used to detect cytokine production from human plasma cells.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report (minimum 10 pages) graded by the supervisor and the course coordinator or the coordinator's delegate (50%).

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply / Next steps: For MIMM: After all parts of this application form are completed, and the hard copy is signed by the professor and the student, bring this application form and your unofficial transcript to Prof. Gregory Marczynski during office hours, who will review/approve as the course coordinator for MIMM 396 (Microbiology) or MIMM 397 (Immunology).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Human subjects; Handling chemicals.