Supervisor's Name: Yojiro Yamanaka
Supervisor's Email: yojiro.yamanaka [at] mcgill.ca
Supervisor's Phone: 514-398-8776
Supervisor's Website: http://blastocyst.mcgill.ca/Yamanaka_lab/Welcome.html
Supervisor's department: Human Genetics
Course number: HGEN 396 (Human Genetics)
Term: Fall 2014-2015
Project start date: Tuesday, September 2, 2014
Project end date: Thursday, December 4, 2014
Project title: Generation of mouse fluorescent reporter lines using CRISPR/Cas9 mediated genome engineering
Project description (50-100 words suggested): The CRISPR/Cas-mediated genome engineering in mouse embryos. Generation of mouse animal models for human diseases is important for current medical research. However, it is costly and time consuming to generate and maintain mouse lines. CRISPR/Cas-mediated genome engineering is a novel genome editing technique to modify mouse and human genomes. The potential of this technique to accelerate generation of animal models has recognized recently. We are introducing this technique in the lab and examining optimal conditions for various genome modifications in the mouse embryo.
Wang et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas9-mediated genome engineering. Cell (2013) 153, 1-9.
Yang et al. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas9-mediated genome engineering. Cell (2013) 1370- 9.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor. Details of the evaluation scheme will be provided by HGEN to supervisors and applicants.
Project status: This project is taken.
How students can apply / Next steps: For HGEN: After all of the parts of this application forms are completed and the hard copy is signed by the professor and the student, bring the application form and a copy of your unofficial transcript to the Department of Human Genetics (to the attention of Dr. Patricia N. Tonin, Stewart Biology Building N5.13) during office hours.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Biohazardous substances.