TLR Regulation of NGF - ANAT 396 Undergraduate Research Project Application Form

Supervisor's Name: Lisbet Haglund

Supervisor's Email: lisbet.haglund [at] mcgill.ca

Supervisor's Phone:

Supervisor's Website:

Supervisor's department: Anatomy and Cell Biology; Surgery

Course number: ANAT 396 (Anatomy and Cell Biology)

Term: Fall 2014-2015

Project start date: Sept. 2, 2014

Project end date: Dec. 4, 2014

Project title: TLR Regulation of NGF

Project description (50-100 words suggested):
Introduction: Intervertebral disc degeneration is the most common cause of low back pain. Nerve growth factor (NGF) is increased in disc degeneration and sensitizes nociceptors, but little is known about its regulation in disc degeneration.
Hypothesis: Toll-like receptor (TLR) activation will increase NGF expression.
Methods: Isolated disc cells will be challenged with PGN (TLR2 agonist) and TLR1, 2 and 6 will be knocked down with siRNA. NGF gene expression and protein will be evaluated.
Significance: NGF is a possible therapeutic target for low back pain and a greater understanding of its regulation could lead to new therapeutic strategies.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% lab work, 50% final report

Project status: This project is taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Human subjects; Biohazardous substances; Handling chemicals.