'Investigating cell choice on multi-protein patterns' - NSCI 396 Undergraduate Research Project Application Form

Supervisor's Name: Tim Kennedy

Supervisor's Email: timothy.kennedy [at] mcgill.ca

Supervisor's Phone: 7136

Supervisor's Website:

Supervisor's department: Anatomy and Cell Biology

Course number: NSCI 396 (Neuroscience)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Investigating cell choice on multi-protein patterns

Project description (50-100 words suggested): Neuron guidance is a key process in development that leads to the creation of the proper connections within the mature nervous system. This process was determined to be monitored by the expression of various guidance proteins that affect the neuron, as it grows, in a variety of ways. In most cases, the understanding behind the mechanism that results in the neuronal response remains unclear. To better study the effects of these molecules on the neurons, a number of techniques have been developed to create protein patterns in vitro. To investigate the role of these proteins, the Micro-and Nano Bioengineering Lab is using microcontact printing (µCP), a stamping technique, to print patterns of a variety of proteins onto glass slides. These micropatterns are then used to investigate the effect on neuronal growth over extended periods of time. The student will generate patterns of various proteins and examine the resulting neural growth response using timelapse microscopy and immunocytochemistry.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Lab notebook 15%, biweekly progress reports, 10%, final report 50%, final presentation 25%

Other project information: Techniques Used:

  • Protein Micropatterning using soft lithography
  • Light microscopy, fluorescence microscopy and live imaging
  • Immunocytochemistry
  • Image analysis (Image J)

Project status: This project is taken.

How students can apply / Next steps: Email sebastien.ricoult [at] mail.mcgill.ca to schedule an interview. This project is now taken.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves Biohazardous substances.