'Effects of Hsp70 mutations on function and CFTR trafficking' - BIOC 396 Undergraduate Research Project Application Form

Supervisor's Name: Jason Young

Supervisor's Email: jason.young2 [at] mcgill.ca

Supervisor's Phone: 514-398-2006

Supervisor's Website:

Supervisor's department: Biochemistry

Course number: BIOC 396 (Biochemistry)

Term: Winter 2013-2014

Project start date: Monday, January 6, 2014

Project end date: Friday, April 11, 2014

Project title: Effects of Hsp70 mutations on function and CFTR trafficking

Project description (50-100 words suggested): Cystic Fibrosis is a genetically driven disease with no known cure, and an ongoing medical challenge. The disease is caused by loss of function of the cystic fibrosis transmembrane conductance regulator, CFTR, a chloride channel critical to maintaining hydration in the lung airways and pancreas. Mutations in CFTR cause it to become misfolded and degraded at the endoplasmic reticulum, instead of trafficking to the cell surface to function. Surprisingly little is known about the chaperone mechanisms that assist wild-type and mutant CFTR folding, particularly by the essential chaperone Hsp70. Our project will characterize mutants of Hsp70 expected to have defects in various biochemical mechanisms, to determine effects on Hsp70 function and CFTR trafficking.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% report, 50% performance

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.