'Photoconversion of Nuclear Probes' - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Claire Brown

Supervisor's Email: claire.brown [at] mcgill.ca

Supervisor's Phone: 541-677-7493

Supervisor's Website: https://mcgill.ca/physiology/directory/core-faculty/claire-brown/

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Photoconversion of Nuclear Probes

Project description (50-100 words suggested): Fluorescent nuclear probes have been shown to convert from fluorescent blue to fluorescent green upon excitation with UV light and 405 nm laser light. This photoconversion can lead to artifacts in images containing green fluorescent labels leading to overestimates of green intensities and false positives for nuclear localization of green fluoescent probes. This project will involve testing different UV and 405 nm excitation powers to determine if there is a critical time or intensity threshold important for this photoconversion from blue to green to occur. The end result will be a set of recommendations for scientists using these blue nuclear dyes so they can ensure they do not induce this photobleaching artifact during standard fluorescence imaging.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% for the written report and 50% for the laboratory work.

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply: Contact me by email and send me a copy of your transcript (unofficial copy is fine).

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.