'Photobleaching rates and pulsed light' - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Claire Brown

Supervisor's Email: claire.brown [at] mcgill.ca

Supervisor's Phone: 541-677-7493

Supervisor's Website: https://mcgill.ca/physiology/directory/core-faculty/claire-brown

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Photobleaching rates and pulsed light

Project description (50-100 words suggested): It has recently been shown that using pulsed laser light reduced photobleaching and phototoxicity in fluorescence microscopy and live cell imaging. This has the benefit of preserving fluorescence during light intensive confocal laser scanning microscope (CLSM) imaging. It also has the benefit of preserving living samples during live cell timelapse imaging. This project will test the dependence of photobleaching of fixed enhanced green fluorescent protein (EGFP) labelled cells with different scanning configurations on the CLSM. The student will learn how to use the confocal microscope, collect images and perform image processing and analysis to extract bleach rates.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% for the laboratory work and 50% for the written report.

Project status: This project is taken.

How students can apply: Contact me by email and send me a copy of your transcript (unofficial copy is fine)

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: None of the above (NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers).