'Functional characterization of endothelial cell in diabetic neuropathy' - NSCI 396 Undergraduate Research Project Application Form

Supervisor's Name: Dr. Ji Zhang

Supervisor's Email: ji.zhang [at] mcgill.ca

Supervisor's Phone: (514) 398-7203, x00036#

Supervisor's Website: PainImmunologyLab.com

Supervisor's department: Microbiology and Immunology; Dentistry

Course number: NSCI 396 (Neuroscience)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Functional characterization of endothelial cell in diabetic neuropathy

Project description (50-100 words suggested): Previous animal studies and patient data have revealed that the vascular system in diabetic nerve is abnormal and damaged. It is also known that inflammation plays a major role in diabetes, as anti-inflammation works to relieve neuropathic pain. In this study, our aim is to show whether vascular abnormalities are related to inflammation in diabetes. We will characterize the function of endothelial cells in diabetic nerve by taking out the rat sciatic nerve, isolating the endothelium, culturing endothelial cells, then extracting the supernatant to measure the chemokine and cytokine levels in both naive and diabetic rats.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor.

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

How students can apply: Bring a printed copy of this application form and your advising transcript to me during office hours.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves handling chemicals.