Supervisor's Name: Colin Crist
Supervisor's Email: colin.crist [at] mcgill.ca
Supervisor's Phone: 514-340-8222 x8541
Supervisor's Website: http://ladydavis.ca/en/colincrist
Supervisor's department: Human Genetics
Course number: HGEN 396 (Human Genetics)
Term: Fall 2013-2014
Project start date: Tuesday, September 3, 2013
Project end date: Tuesday, December 3, 2013
Project title: Post-transcriptional mechanisms regulating skeletal muscle stem cell activity
Project description (50-100 words suggested): Tissue specific stem cells ensure the regeneration of adult tissues following injury. These stem cells must also self-renew, such that they are present throughout the lifetime of the individual. Our laboratory uses genetic tools in the mouse to study the mechanism by which skeletal muscle stem cells self-renew.
We have learned that self-renewal of skeletal muscle stem cells requires the microRNA pathway in cooperation with the phosphorylation of FMRP and eIF2alpha proteins. In this HGEN396 project, the student will be responsible for the analysis of skeletal muscle stem cell activity when one of these mechanisms is manipulated. While obtaining a good introduction to the field of stem cell biology, the student may learn techniques in cell isolation and culture, immunolabeling/fluorescence microscopy and molecular biology.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory (or equivalent) performance (40%), a final written report (50%), and an oral presentation (10%) by the supervisor. Details of the evaluation scheme will be provided by HGEN to supervisors and applicants.
Other project information:
- Please consider that our laboratory is at the Lady Davis Institute for Medical Research at the Jewish General Hospital. 3755 Côte Ste-Catherine Road, Montreal, QC.
- The project description above is general. Specific details will be decided after discussion with the student.
Project status: This project is open to applicant.
How students can apply: After all of the parts of this application forms are completed and the hard copy is signed by the professor and the student, bring the application form and a copy of your unofficial transcript to the Department of Human Genetics (to the attention of Dr. Patricia N. Tonin, Stewart Biology Building N5.13) during office hours.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Animal subjects; Handling chemicals.