'Comparative evaluation of inhibitors of Inositol Requiring Enzyme 1' - BIOC 396 Undergraduate Research Project Application Form

Supervisor's Name: Dr. David Thomas

Supervisor's Email: david.thomas [at] mcgill.ca

Supervisor's Phone: 2973

Supervisor's Website:

Supervisor's department: Biochemistry

Course number: BIOC 396 (Biochemistry)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Comparative evaluation of inhibitors of Inositol Requiring Enzyme 1.

Project description (50-100 words suggested): Inositol Requiring Enzyme 1 (IRE1) is an important stress transducer that is required for cellular homeostasis when the protein folding capacity of the endoplasmic reticulum is challenged.   Recent evidence has shown that IRE1 signaling is essential for the survival of multiple myeloma cancer cells and thus represents an emerging therapeutic target. We have identified a small number of candidate IRE1 inhibitors that require further characterization. The student will use established assays to monitor the kinase and endoribonuclease activities of IRE1 in order to determine the mechanism of action and potency of IRE1 inhibitors. The anti-myeloma properties of the most potent IRE1 inhibitors will be evaluated using a variety of viability assays performed on treated human multiple myeloma cell lines.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report graded by the supervisor and the course coordinator (50%).

Project status: This project is taken. The professor has no more '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves handling chemicals.