'Functional study of PKLR mutations and association with malaria resistance' - BIOC 396 Undergraduate Research Project Application Form

Supervisor's Name: Philippe Gros

Supervisor's Email: philippe.gros [at] mcgill.ca

Supervisor's Phone: 514-398-7291

Supervisor's Website: https://mcgill.ca/biochemistry/about-us/department/faculty-members/gros

Supervisor's department: Biochemistry

Course number: BIOC 396 (Biochemistry)

Term: Fall 2013-2014

Project start date: Tuesday, September 3, 2013

Project end date: Tuesday, December 3, 2013

Project title: Functional study of PKLR mutations and association with malaria resistance

Project description (50-100 words suggested): PKLR encodes for the erythrocyte-specific isoform of the pyruvate kinase (PK-R) enzyme. Mutations that alter the functional activity of this enzyme result in PK-deficiency, the most common cause of nonspherocytic hemolytic anemia. Recently, PK-deficiency was associated with resistance to malaria. Several PKLR mutations were identified within two populations living in malaria endemic regions. By examining the effect of these mutations on PK-R activity, we seek to determine whether these mutations have a deleterious effect on enzyme function resulting in PK-deficiency, which may be associated with resistance to malaria.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% Laboratory performance, 50% Written report

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves handling chemicals.