'3DEM reconstruction of polyomavirus' - ANAT 396 Undergraduate Research Project Application Form

Supervisor's Name: Isabelle ROUILLER

Supervisor's Email: isabelle.rouiller [at] hotmail.com

Supervisor's Phone: 3244

Supervisor's Website: https://mcgill.ca/rouiller-lab/

Supervisor's department: Anatomy and Cell Biology

Course number: ANAT396 (Anatomy and Cell Biology)

Term: Fall 2012-2013

Project start date: Tuesday, September 4, 2012

Project end date: Tuesday, December 4, 2012

Project title: 3DEM reconstruction of polyomavirus

Project description: Cell entry of non-enveloped viruses is poorly understood. To understand how polyomavirus enter the cell, we are studying a possible conformational change the capsid undergoes before cell entry using cryo-electron microscopy and single particle reconstruction. Two images data sets will be available, one of polyomavirus bound to sugar, the other of polyomavirus in the absence of sugar. The student will be responsible for calculating 3D EM maps with both data sets. He will select the particles from the images, CTF correct them and calculate the 3D reconstruction. All images processing methods will be done using standard protocol in use in the laboratory. It is expected that the student will write a detailed protocol of the work performed, present his work in a group meeting as well as write a summary of the work performed.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% final report; 20% oral presentation; 30% attendance and motivation.

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

How students can apply: N/A; this project is filled.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.