Green Light Microscopy Light Source Testing - PHGY 396 Undergraduate Research Project Application Form

Supervisor's Name: Claire Brown

Supervisor's Email: claire.brown [at]

Supervisor's Phone: 00795

Supervisor's Website:

Supervisor's department: Physiology

Course number: PHGY 396 (Physiology)

Term: Winter 2014-2015

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: Green Light Microscopy Light Source Testing

Project description (50-100 words suggested): There is a critical need for sustainable and minimal environmental impact of our research infrastructure. New LED based light source technologies are quickly replacing mercury based light sources for fluorescence microscopy. There is very little concrete information from manufacturers on the environmental impact of these technologies and how they perform in the research setting. Therefore, there is a need to test and validate the performance and the environmental impact of these light sources. The project will involve learning how to use a wide-field light microscope and a light power meter. Light sources will be tested for power stability on different time scales to ensure high quality quantitative scientific data will be collected on the microcopes. Testing the light source power consumption under different operating conditions will determine if the systems do in fact provide a lower power consumption than the former mercury based technologies. Photo-toxicity of different settings of the light source on living samples will be determined by performing live cell migration assays.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% written report; 50% laboratory activity.

Project status: This project is taken.

How students can apply / Next steps: email me the project title you are interested in and your up to date CV and unofficial transcript.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.