Supervisor's Name: Claire Brown
Supervisor's Email: claire.brown [at] mcgill.ca
Supervisor's Phone: 00795
Supervisor's Website: www.mcgill.ca/abif
Supervisor's department: Physiology
Course number: PHGY 396 (Physiology)
Term: Winter 2014-2015
Project start date: Friday, September 4, 2015
Project end date: Monday, December 7, 2015
Project title: 3D Super-resolution Quality Control
Project description (50-100 words suggested): Imaging biological samples in 3D has become very routine and quantifying the fluorescence intensities that come from these images is central to our understanding of biology. Proper testing of equipment is required to ensure that images coming from the microscope are quantitative. However, there are very few standard tests and standard samples for 3D imaging. This project will involved the development and testing of a 3D standard sample on a super resolution fluorescence microscope. The project will involve learning about structured illumination microscopy. Preparation of 3D fluorescent microsphere calibration slides. Imaging on the super resolution microscope and 3D image analysis to determine if the system has a linear response and if so if that linear response if maintained when imaging 100 um deep into samples. This information will be used to develop a standard protocol for sample preparation and analysis. Once they are finalized the sample and protocol will be used in regular testing of the superresolution microscope.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): 50% written report; 50% laboratory activity.
Project status: This project is taken.
How students can apply / Next steps: email me the project title you are interested in and your up to date CV and unofficial transcript.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves NEITHER animal subjects, nor human subjects, nor biohazardous substances, nor radioactive materials, nor handling chemicals, nor using lasers.