Characterization of epigenetically active loci in response to di(2-ethylhexyl) phthalate - PHAR 396 Undergraduate Research Project Application Form

Supervisor's Name: Vassilios Papadopoulos

Supervisor's Email: vassilios.papadopoulos [at] mcgill.ca

Supervisor's Phone: 514-934-1934 ext. 44580

Supervisor's Website:

Supervisor's department: Pharmacology

Course number: PHAR 396 (Pharmacology)

Term: Fall 2015-2016

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: Characterization of epigenetically active loci in response to di(2-ethylhexyl) phthalate

Project description (50-100 words suggested): Di(2-thylhexyl) phthalate (DEHP) is a plasticizer widely used in consumer products. In utero exposure to DEHP results in decreased aldosterone levels in the adult male offspring. The data suggest that the long-term endocrine effects of DEHP are mediated by an epigenetic mechanism since DEHP is cleared soon after birth. We previously identified several differentially methylated CpGs (dmCpG) in the adrenal gland induced by DEHP using a genome-wide methylation technique. The goal of this project is to use reporter constructs to characterize the sensitivity of the dmCpG loci to DEHP. The outcome of this work will help to further understand gene-environment interactions.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Written report for 50% and 50% based on performance in the lab

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.