Dimerization of Aplysia californica classical calpain in memory formation - NSCI 396 Undergraduate Research Project Application Form

Supervisor's Name: Wayne Sossin

Supervisor's Email: wayne.sossin [at] mcgill.ca

Supervisor's Phone:

Supervisor's Website:

Supervisor's department: Psychology

Course number: NSCI 396 (Neuroscience)

Term: Fall 2015-2016

Project start date: Tuesday, September 8, 2015

Project end date: Monday, December 7, 2015

Project title: Dimerization of Aplysia californica classical calpain in memory formation

Project description (50-100 words suggested): Persistently active kinases maintain memories. One mechanism for the production of these kinases is proteolysis of protein kinases to remove their regulatory domains. This proteolysis occurs by the family of intracellular proteases called calpains in a model system for examining memory, Aplysia californica. How calpains are activated during memory formation is not known. One important issue about calpain activation is whether or not calpains generate homo or heterodimers through their EF hand domain. The student will examine this question using standard molecular biology methods to generate constructs and biochemistry methods to study protein-protein interactions.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on laboratory performance as evaluated by the research supervisor (50%) and the final written research report graded by the supervisor (50%).

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.