Supervisor's Name: Maria Pompeiano
Supervisor's Email: maria.pompeiano [at] mcgill.ca
Supervisor's Phone: 514-398-6080
Supervisor's department: Psychology
Course number: NSCI 396 (Neuroscience)
Term: Fall 2015-2016
Project start date: Friday, September 4, 2015
Project end date: Monday, December 7, 2015
Project title: TRPC5 and brain maturation in the chick embryo
Project description (50-100 words suggested): TRPC5 is a Ca2+-permeable cation channels which is abundant in the brain where it act as a cellular sensor for diverse stimuli (Puram et al 2011). It has been shown to keep certain neuronal populations depolarized and thus tonically firing in adult rodents (Cvetkovic-Lopes et al 2011).
TRPC5 is also expressed during brain development in mammals where it seems to control dendrite patterning (Puram et al 2011).
Preliminary observations in the lab indicated that TRPC5 is expressed in various brain areas of the chick embryo starting from early ages. Embryos 10-20 day old as well as newborn chicks (n=1 for each of 5 ages) were qualitatively examined. These data suggests that TRPC5 may play a role in the development of activity and maturation of different neural networks.
The present project aims at extending these qualitative observations to one additional series of animals (n=1 for each of 5 ages), which will be stained by using the immunohistochemical protocol established in the lab. In addition, quantitative data will be obtained from a limited number of significant areas in all animals (n=6 for each age) by using the Stereo Investigator software and a Leica DM6000 B microscope.
Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.
Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory performance (50%) and a final written report (50%) by the supervisor.
Project status: This project is taken. The professor has no more '396' projects this term.
Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.