cFos expression in the chick brain - NSCI 396 Undergraduate Research Project Application Form

Supervisor's Name: Maria Pompeiano

Supervisor's Email: maria.pompeiano [at] mcgill.ca

Supervisor's Phone: 514-398-6080

Supervisor's Website:

Supervisor's department: Psychology

Course number: NSCI 396 (Neuroscience)

Term: Fall 2015-2016

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: cFos expression in the chick embryo brain

Project description (50-100 words suggested): cFos protein is an immediate-early gene product and a transcription factor. It is induced in neurons by a variety of factors including depolarizing stimuli, cytosolic Ca2+ or cAMP increase, and growth factor stimulation. Although cFos induction is not always associated with increased firing rate, it is certainly indication of recent neuronal stimulation. cFos has thus extensively been used as a marker of neuronal activation. Not much detailed information is present in the literature about developmental expression of cFos in any animal species (Caubet 1989; Gubits et al. 1988; Parma et al. 1991). We are here proposing to study cFos expression in the brain of chick embryos of different ages (E6-E20) by using colorimetric immunohistochemistry. We expect to find early activation in the areas controlling neuroendocrine and autonomic functions. This study will give some hints about the maturation order of different neuronal network.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): Final grade shall be based on an evaluation of laboratory performance (50%) and a final written report (50%) by the supervisor.

Project status: This project is taken. The professor has no more '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.