Hoogsteen Basepairs in Duplex DNA - CHEM 396 Undergraduate Research Project Application Form

Supervisor's Name: Anthony Mittermaier

Supervisor's Email: anthony.mittermaier [at] mcgill.ca

Supervisor's Phone: 5143983085

Supervisor's Website: https://mcgill.ca/mittermaierlab/

Supervisor's department: Chemistry

Course number: CHEM 396 (Chemistry)

Term: Fall 2015-2016

Project start date: Friday, September 4, 2015

Project end date: Monday, December 7, 2015

Project title: Hoogsteen Basepairs in Duplex DNA

Project description (50-100 words suggested): It has recently been shown by dynamic NMR spectroscopy that duplex DNA undergoes transient excursions from canonical Watson-Crick base-pairing to Hoogsteen conformations, which are likely important in DNA modification and repair processes. Our lab has developed a high-throughput approach to characterize this process through studying the stability of chemically modified DNA. This project involves testing the population of Hoogsteen intermediates as a function of buffer conditions in order to better understand the behaviour inside living cells.

Prerequisite: 1 term completed at McGill + CGPA of 3.0 or higher; or permission of instructor.

Grading scheme (The final report must be worth at least 50% of final grade): 50% performance / 50% final report

Project status: This project is taken; however students may contact the professor to discuss other possible '396' projects this term.

Ethics, safety, and training: Supervisors are responsible for the ethics and safety compliance of undergraduate students. This project involves: Handling chemicals.